AI Article Synopsis
- This study focused on identifying fungal endophytes from six orchid species in southeastern Queensland using methods like ITS-PCR and RFLP analysis, revealing five main fungal groupings.
- The research indicated that the endophytes of three Pterostylis species likely belong to the Thanatephorus genus, while a fungus from Acianthus spp. showed a close genetic relationship to Epulorhiza repens.
- Findings also suggested that fungal partners exhibit specificity within the orchid genera Acianthus, Caladenia, and Pterostylis, with some species showing different colonization abilities.
Article Abstract
We used ITS-RFLP and sequence analysis to determine the identities of the fungal endophytes of six terrestrial orchid species from southeastern Queensland, a region previously unexplored in this context. Pure cultures of orchid--colonising fungi were obtained and fungal identities were assessed by means of ITS-PCR, RFLP analysis, sequence comparison, and protocorm colonisation tests. ITS-PCR and RFLP analysis resulted in five main groupings. Sequencing and GenBank comparison of these five groups showed that the fungal endophytes isolated from the three Pterostylis species were probably Thanatephorus species. There was close sequence identity (90%) of the fungus isolated from Acianthus spp. to Epulorhiza repens, suggesting these may be the same fungal species. However, that only E. repens succeeded in colonising protocorms of Thelymitra pauciflora suggests these may be different species of Epulorhiza. Analysis of the ITS and LSU sequences of the fungus isolated from Caladenia carnea showed high identities with a sequence from a Sebacina vermifera originally isolated from Caladenia dilatata. These results show that there is specificity for fungal partners within the orchid genera Acianthus, Caladenia and Pterostylis.
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