A TRP5-based reversion system that allows the rates of all possible base pair substitutions to be measured when the TRP5 locus is in both orientations relative to a defined origin of replication has been developed. This system should be useful for a wide variety of mutation and repair studies in yeast.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1451166PMC
http://dx.doi.org/10.1534/genetics.105.042697DOI Listing

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