Background: The aim of the present study was to examine the presence of motilin in the duodenal epithelial cells of premature infants of < 32 weeks gestation.
Methods: Specimens from 10 deceased infants (gestational age: 26.4 +/- 2.7 weeks and birthweight: 808 +/- 303 g) were examined as subjects. All infants died of severe cardiopulmonary disorder or intraventricular hemorrhage (grade IV). The average survival period was 3.1 +/- 1.9 days. Autopsies were performed and formalin-fixed duodenums were immunostained with rabbit antiserum to motilin by the labeled streptavidin-biotin (LSAB) method. An adult duodenum obtained by pancreatoduodenectomy was also examined for the presence of motilin as a positive control specimen. An absorption test using motilin peptide was performed to prove the specificity of the binding with rabbit antiserum to motilin.
Results: Motilin-containing cells were detected in the adult specimen, and the binding by rabbit antiserum to motilin was completely inhibited by excess amounts of motilin peptide, indicating that this binding was specific to motilin. All 10 infants had presence of motilin antigen in the epithelial cells of their duodenums.
Conclusion: This preliminary study indicates that the immunohistological analysis is specific to detect motilin-containing cells, and certifies the presence of motilin in duodenal epithelial cells of premature infants of < 32 weeks gestation, including one at only 22 weeks gestation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1442-200x.2005.02050.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Zuojin Pill enhances gastrointestinal motility by modulating the pacemaker potentials in interstitial cells of Cajal through multiple signaling pathways.
Int J Med Sci
December 2024
Department of Longevity and Biofunctional Medicine, School of Korean Medicine, Pusan National University, Yangsan 50612, Republic of Korea.
Article Synopsis
- Zuojin Pill (ZJP) is a traditional herbal remedy aimed at treating gastrointestinal disorders, and the study explores how it affects interstitial cells of Cajal (ICCs) and gut movement in mice.
- The research found that ZJP depolarizes ICCs, reducing their pacemaker activity and affecting calcium signaling, which is crucial for normal GI motility; this process is influenced by specific receptor interactions and various signaling pathways.
- Additionally, ZJP improved intestinal transit in mouse models of GI motility disorders and increased levels of key substances that promote gut movement, suggesting its potential as a therapeutic agent in digestive health.
The probiotic fermented milk of Lacticaseibacillus paracasei JY062 and Lactobacillus gasseri JM1 alleviates constipation via improving gastrointestinal motility and gut microbiota.
J Dairy Sci
April 2024
Key Laboratory of Dairy Science, Ministry of Education, College of Food Science and Engineering, Northeast Agricultural University, Harbin 150030, China. Electronic address:
Constipation is directly related to the intestinal microenvironment, in which the promotion of gastrointestinal (GI) motility and improvement of gut microbiota distribution are important for alleviating symptoms. Herein, after the intervention of probiotic fermented milk (FMMIX) containing Lacticaseibacillus paracasei JY062 and Lactobacillus gasseri JM1 for 14 d in Kunming mice with loperamide-induced constipation, the results indicated that FMMIX significantly increased the secretion of serum motilin, gastrin and 5-hydroxytryptamine, as well as decreased the secretion of peptide YY, vasoactive intestinal peptide, and nitric oxide in mice. As determined by immunohistochemical analysis, FMMIX promoted an augmentation in the quantity of Cajal interstitial cells.
View Article and Find Full Text PDFSerotonin, ghrelin, and motilin gene/receptor/transporter polymorphisms in childhood functional constipation.
Rev Assoc Med Bras (1992)
March 2023
Istanbul Haseki Training and Research Hospital, Department of Pediatric Gastroenterology - Istanbul, Turkey.
Objective: Functional constipation is the most common form of constipation, and its exact aetiology is still unclear. However, it is known that deficiencies in hormonal factors cause constipation by changing physiological mechanisms. Motilin, ghrelin, serotonin acetylcholine, nitric oxide, and vasoactive intestinal polypeptide are factors that play a role in colon motility.
View Article and Find Full Text PDFAim: To establish the role of the main risk factors and endocrine cells of the antrum of the stomach producing motilin (M-cells) in the occurrence of cholelithiasis.
Materials And Methods: The first group included 122 patients with cholelithiasis. The second group consisted of 30 healthy individuals who underwent medical examination.
Grape seed powder increases gastrointestinal motility.
Int J Med Sci
June 2022
Division of Longevity and Biofunctional Medicine, Pusan National University School of Korean Medicine, Yangsan 50612, Republic of Korea.
Grape seed is an important natural bioactive product with various health benefits. Interstitial cells of Cajal (ICCs) are pacemaker cells in the gastrointestinal (GI) tract. The present study investigated the effects of grape seed powder (GSP) on ICC properties and GI motility.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!