By its numerous properties and importance in cheese technology (production of colour, flavour, bacteriocins and resistance to salt) Brevibacterium linens is a major cheese ripening bacteria. However, the genetic approach of such biological functions has been hindered, up to now, by the lack of tools necessary to realise genetic modifications in this species. Our objective was to demonstrate that it is possible to genetically modify several strains exhibiting interesting technological properties, especially the production of sulphur compounds. We worked with a phenotypically and genetically diverse collection of 11 strains. We genetically transformed several Brevi. linens with acceptable rates with plasmids classically used to transform lactic acid bacteria and other Gram+ bacteria. These results open up new prospects to investigate the most interesting Brevi. linens metabolic pathways both at the biochemical and genetic level.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1017/s0022029905000737 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Oral administration of Brevibacterium linens from washed cheese increases the proportions of short-chain fatty acid-producing bacteria and lactobacilli in the gut microbiota of mice.
Anim Sci J
December 2023
Department of Biomolecular Innovation, Institute for Biomedical Sciences, Shinshu University, Nagano, Japan.
Brevibacterium linens (B. linens) is a dairy microorganism used in the production of washed cheese. However, there has been little research on B.
View Article and Find Full Text PDFModification and Production of Encapsulin.
Methods Mol Biol
June 2023
Department of Molecules and Materials, MESA+ Institute for Nanotechnology, University of Twente, Enschede, The Netherlands.
Encapsulins are a class of protein nanocages that are found in bacteria, which are easy to produce and engineer in E. coli expression systems. The encapsulin from Thermotoga maritima (Tm) is well studied, its structure is available, and without modification it is barely taken up by cells, making it promising candidates for targeted drug delivery.
View Article and Find Full Text PDFOily bilge water treatment using indigenous soil bacteria: Implications for recycling the treated sludge in vegetable farming.
Chemosphere
September 2023
Department of Botany & Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia.
Hydrocarbon contamination from motorized vessels operating on seas threaten marine ecosystems and need to treated efficiently. A bilge wastewater treatment using indigenous bacteria isolated from oil contaminated soil was studied. Five bacterial isolates (Acinetobacter baumanni, Klebsiella aerogenes, Pseudomonas fluorescence, Bacillus subtilis and Brevibacterium linens) were isolated from port soil and used in the bilge water treatment.
View Article and Find Full Text PDFScreening of Structurally Distinct Lycopene β-Cyclases for Production of the Cyclic C40 Carotenoids β-Carotene and Astaxanthin by .
J Agric Food Chem
May 2023
Genetics of Prokaryotes, Faculty of Biology and CeBiTec, Bielefeld University, 33615 Bielefeld, Germany.
Lycopene β-cyclase (EC 5.5.1.
View Article and Find Full Text PDFInvert emulsions alleviate biotic interactions in bacterial mixed culture.
Microb Cell Fact
January 2023
LIBio, Université de Lorraine, Nancy, France.
The large application potential of microbiomes has led to a great need for mixed culture methods. However, microbial interactions can compromise the maintenance of biodiversity during cultivation in a reactor. In particular, competition among species can lead to a strong disequilibrium in favor of the fittest microorganism.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!