New principle of electromagnetophoretic adsorption-desorption microchromatography.

J Chromatogr A

Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan.

Published: May 2005

AI Article Synopsis

  • A new method has been developed for separating micrometer-sized particles in liquids by manipulating electromagnetophoretic forces within a capillary flow system.
  • The technique combines Stokes force from bulk flow with the adsorption-desorption force on the capillary walls, using alternating current and a magnetic field.
  • Successful separation of polystyrene particles with diameters of 10 and 20 micrometers was achieved in a short fused-silica capillary under high magnetic field conditions.

Article Abstract

A new principle for the chromatographic micro-separation of micrometer-sized particles in liquid has been invented by switching the electromagnetophoretic force in a capillary flow system. The principle is the combination of the Stokes force by the bulk flow and the adsorption-desorption force on a capillary inner surface controlled by an electromagnetophoretic buoyancy generated by an alternative current and a homogenous magnetic field. The observed retention profiles of test micro-particles was explained by the "zigzag" migration model mainly depended on particle size and their adsorption force to the capillary wall. By this method, we could succeed to separate polystyrene particles of 10 microm and 20 microm in diameter dispersed in 1 M KCl solution containing 0.01% Triton X-100 using only 1 mm long fused-silica capillary under 10T.

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Source
http://dx.doi.org/10.1016/j.chroma.2004.10.059DOI Listing

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