The chick midbrain is subdivided into functionally distinct ventral and dorsal domains, tegmentum and optic tectum. In the mature tectum, neurons are organized in layers, while they form discrete nuclei in the tegmentum. Dorsoventral (DV) specification of the early midbrain should thus play a crucial role for the organization of the neuronal circuitry in optic tectum and tegmentum. To investigate regional commitment and establishment of cellular differences along the midbrain DV axis, we examined the commitment of gene expression patterns in isolated ventral and dorsal tissue in vivo and in vitro, and studied their cell mixing properties. Use of explant cultures, and grafting of dorsal midbrain into a ventral environment or vice versa, revealed a gradual increase in the autonomy of region-specific gene regulation between stages 12 and 18 (embryonic day 2 to 3). This process becomes independent of the activity of midline organizers, such as floor and roof plate, by stage 16. Once the DV axis polarity is fixed, cells from dorsal and ventral midbrain adopt differential adhesive properties. Thus between stages 18 to 23 (embryonic day 3 and 4), cells of dorsal and ventral origin start to separate from each other, at a time-point when the majority of midbrain cells is not yet differentiated. Hence, our results suggest that progressive specification of the midbrain DV axis is accompanied by progressively reduced cell mixing between dorsal and ventral precursors, leading to a partial regionalization of midbrain tissue into autonomous units of precursor cell populations.

Download full-text PDF

Source
http://dx.doi.org/10.1002/dvdy.20434DOI Listing

Publication Analysis

Top Keywords

dorsal ventral
12
midbrain
9
ventral dorsal
8
optic tectum
8
midbrain axis
8
cell mixing
8
stages embryonic
8
embryonic day
8
cells dorsal
8
ventral
6

Similar Publications

Hypomanic personality traits (HPT) are susceptibility markers for psychiatric disorders, particularly bipolar disorder, and are strongly associated with aggressive behaviors. However, the neuropsychological mechanisms underlying this association remain unclear. This study utilized psychometric network analysis and (IS-RSA) to explore the neuropsychological circuits that link HPT to aggression in a large non-clinical population.

View Article and Find Full Text PDF

Swimmers propel their bodies forward by generating vortices around themselves, which produce fluid force during underwater undulatory swimming (UUS). This study aimed to investigate the propulsive and braking contributions of the vortices of the lower limbs, trunk, and upper limbs during UUS. The kinematic data and three-dimensional digital model were collected from nine male swimmers.

View Article and Find Full Text PDF

Abnormal network homogeneity in patients with bipolar disorder in attention network.

Brain Imaging Behav

January 2025

Key Laboratory of Adolescent Cyberpsychology and Behavior (Ministry of Education), Wuhan, China.

Bipolar disorder (BD) is a complex psychiatric condition marked by significant mood fluctuations that deeply affect quality of life. Understanding the neural mechanisms underlying BD is critical for improving diagnostic accuracy and developing more effective treatments. This study utilized resting-state functional magnetic resonance imaging (rs-fMRI) to investigate functional connectivity within the ventral and dorsal attention networks in 52 patients with BD and 51 healthy controls.

View Article and Find Full Text PDF

The hippocampus has a known role in learning and memory, with the ventral subregion supporting many learning tasks involving affective responding, including fear conditioning. Altered neuronal intrinsic excitability reflects experience-dependent plasticity that supports learning-related behavioral changes. Such changes have previously been observed in the dorsal hippocampus following fear conditioning, but little work has examined the effect of fear conditioning on ventral hippocampal intrinsic plasticity.

View Article and Find Full Text PDF

Cortical thickness analyses have provided valuable insights into changes in cortical brain structure after stroke and their association with recovery. Across studies though, relationships between cortical structure and function show inconsistent results. Recent developments in diffusion-weighted imaging of the cortex have paved the way to uncover hidden aspects of stroke-related alterations in cortical microstructure, going beyond cortical thickness as a surrogate for cortical macrostructure.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!