Background: Serum total magnesium (tMg) consists of 3 fractions: ionized magnesium (iMg), protein-bound magnesium (pbMg), and complexed magnesium (cMg). Serum iMg may be measured by an ion-selective electrode, but determination of pbMg and cMg has not been attempted in dogs.
Objectives: The objectives of this study were to assess the validity of a micropartition system to fractionate serum tMg and to establish reference intervals for pbMg, cMg, and iMg in clinically normal dogs using this method.
Methods: Serum samples from 10 clinically healthy dogs were fractionated using a micropartition system (Centrifree YM-30, Amicon Corp, Lexington, MA, USA). Serum tMg and iMg were measured in whole serum, and tMg was also measured in the ultrafiltrate. Concentration of cMg was obtained by the subtraction of iMg from tMg concentrations of the ultrafiltrate. Protein-bound Mg was calculated by subtracting the tMg concentration of the ultrafiltrate from the tMg concentration of whole serum.
Results: Results for pbMg and cMg using the micropartition system showed good reproducibility. Determination of tMg and iMg had acceptable inter- and intra-assay precision. Concentrations of iMg, cMg, and pbMg were 0.50 +/- 0.05 mmol/L, 0.05 +/- 0.04 mmol/L, and 0.24 +/- 0.04 mmol/L, representing 63%, 6%, and 31% of the tMg concentration, respectively.
Conclusions: The micropartition system was a reproducible means to accurately assess cMg and pbMg concentrations in dogs.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1939-165x.2005.tb00026.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Non-linear plasma protein binding of cannabidiol.
J Cannabis Res
June 2024
Department of Biomedical and Pharmaceutical Sciences, Touro College of Pharmacy, 3 Times Square, New York, NY, 10036, USA.
Background: Cannabidiol is highly bound to plasma proteins. Changes in its protein binding can lead to altered unbound plasma concentrations and result in alteration of pharmacological activity of cannabidiol-containing medications. This research has assessed non-linearity of cannabidiol plasma protein binding and the potential effect of tizoxanide on the binding.
View Article and Find Full Text PDFSerum protein binding of 25 antiepileptic drugs in a routine clinical setting: A comparison of free non-protein-bound concentrations.
Epilepsia
July 2017
Department of Clinical & Experimental Epilepsy, NIHR University College London Hospitals Biomedical Research Centre, UCL Institute of Neurology, London, United Kingdom.
Objective: Given that only the free non-protein-bound concentration of an antiepileptic drug (AED) crosses the blood-brain barrier, entering the brain and producing an antiepileptic effect, knowledge and measurement of the free drug fraction is important. Such data are sparse, particularly for newer AEDs, and have arisen from the use of disparate methodologies and settings over the past six decades. We report on the protein binding of 25 AEDs that are available for clinical use, along with two pharmacologically active metabolites (carbamazepine-epoxide and N-desmethyl clobazam), using standardized methodology and under set conditions.
View Article and Find Full Text PDFSerum concentrations of calcium, phosphorus, magnesium and calciotropic hormones in donkeys.
Am J Vet Res
August 2006
Department of Medicina y Cirugia Animal, Universidad de Cordoba, Campus Universitario Rabanales, Ctra Madrid-Cadiz km 396, 14014 Cordoba, Spain.
Objective: To provide reference values for serum biochemical variables that are used for evaluation of mineral metabolism in donkeys and compare values with those in horses.
Animals: 18 donkeys and 18 horses.
Procedures: Total calcium (tCa), total magnesium (tMg), and inorganic phosphorus (P) concentrations were measured in serum samples via spectrophotometry.
Fractionation of calcium and magnesium in equine serum.
Am J Vet Res
March 2006
Department of Medicina y Cirugia Animal, Universidad de Cordoba, Campus Universitario Rabanales, Ctra Madrid-Cadiz km 396, 14014 Cordoba, Spain.
Objective: To establish reference values for protein-bound, ionized, and weak-acid complexed fractions of calcium and magnesium in equine serum and determine stability of ionized calcium (iCa) and ionized magnesium (iMg) in serum samples kept under various storage conditions.
Animals: 28 clinically normal horses.
Procedure: Total calcium (tCa) and magnesium (tMg) in equine serum were fractionated by use of a micropartition system that allows separation of protein-bound calcium (pCa) and magnesium (pMg) and ultrafiltrable calcium (microCa) and magnesium (microMg) fractions.
Fractionation of canine serum magnesium.
Vet Clin Pathol
June 2005
Diagnostic Center for Population and Animal Health, Endocrinology Section, Michigan State University, Lansing, MI, USA.
Background: Serum total magnesium (tMg) consists of 3 fractions: ionized magnesium (iMg), protein-bound magnesium (pbMg), and complexed magnesium (cMg). Serum iMg may be measured by an ion-selective electrode, but determination of pbMg and cMg has not been attempted in dogs.
Objectives: The objectives of this study were to assess the validity of a micropartition system to fractionate serum tMg and to establish reference intervals for pbMg, cMg, and iMg in clinically normal dogs using this method.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!