To determine whether AlgL's lyase activity is required for alginate production in Pseudomonas aeruginosa, an algLdelta::Gm(r) mutant (FRD-MA7) was created. algL complementation of FRD-MA7 restored alginate production, but algL constructs containing mutations inactivating lyase activity did not, demonstrating that the enzymatic activity of AlgL is required for alginate production.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1112040 | PMC |
| http://dx.doi.org/10.1128/JB.187.11.3869-3872.2005 | DOI Listing |
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