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Tailed molecular beacon probes: an approach for the detection of structured DNA and RNA analytes.
Chem Commun (Camb)
January 2025
Chemistry Department, University of Central Florida, Orlando, Florida 32816, USA.
Molecular beacon (MB) probes have been extensively used for nucleic acid analysis. However, MB probes fail to hybridize with folded DNA or RNA. Here, we demonstrate that MB probes equipped with extra sequences complementary to the analyte, named 'tail', can increase the signal-to-background ratio by ∼40-fold and hybridization rates by ∼800-fold compared to conventional MB probes.
View Article and Find Full Text PDFBurnout Syndrome in forensic medicine and its association with vicarious trauma, posttraumatic stress syndrome and occupational stress.
Int J Legal Med
January 2025
Faculty of Medicine, Lucian Blaga University of Sibiu, Sibiu, 550169, Romania.
The burnout phenomenon is a subject of considerable interest due to its impact on both employee well-being and scientific inquiry. Workplace factors, both intrinsic and extrinsic, play a pivotal role in its development, often leading to job dissatisfaction and heightened burnout risk. Chronic stress and burnout induce significant dysregulation in the autonomic nervous system and hormonal pathways, alongside structural brain changes.
View Article and Find Full Text PDFContinuous increase in podocyte numbers in the first 36 months of life-insights from forensic autopsies in Japanese children.
Pediatr Nephrol
January 2025
Department of Pediatrics, Shiga University of Medical Science, Shiga, 520-2192, Japan.
Background: Podocyte depletion is a critical factor in glomerulosclerosis development. While podocyte numbers per glomerulus typically decline with age in adults, they are hypothesized to increase during childhood. However, studies on podocyte number progression in childhood have been limited.
View Article and Find Full Text PDFMonitoring the trends of carbon monoxide and tropospheric formaldehyde in Edo State using Sentinel-5P and Google Earth Engine from 2018 to 2023.
Environ Monit Assess
January 2025
Laboratory for Ecotoxicology and Environmental Forensics, University of Benin, PMB 1154, Benin City, Nigeria.
This research was carried out to assess the concentrations of carbon monoxide (CO) and formaldehyde (HCHO) in Edo State, Southern Nigeria, using remote sensing data. A secondary data collection method was used for the assessment, and the levels of CO and HCHO were extracted annually from Google Earth Engine using information from Sentinel-5-P satellite data (COPERNISCUS/S5P/NRTI/L3_) and processed using ArcMap, Google Earth Engine, and Microsoft Excel to determine the levels of CO and HCHO in the study area from 2018 to 2023. The geometry of the study location is highlighted, saved and run, and a raster imagery file of the study area is generated after the task has been completed with a 'projection and extent' in the Geographic Tagged Image File Format (.
View Article and Find Full Text PDFCorrection to "Fluorescence Sensing Technologies for Ophthalmic Diagnosis".
ACS Sens
January 2025
Department of Chemical Engineering, Imperial College London, South Kensington, London SW7 2BU, United Kingdom.
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