AI Article Synopsis
- The Scatchard plot of epidermal growth factor (EGF) binding to the EGF receptor (EGFR) exhibits a concave up shape, which has puzzled researchers for over a decade.
- Previous studies suggested that negative cooperativity in binding or the presence of external binding sites could explain this phenomenon, but those explanations weren't universally accepted.
- This new research proposes that the uneven distribution of EGFR on the plasma membrane plays a significant role in creating the concave up shape observed in the Scatchard plot.
Article Abstract
The mechanism responsible for the concave up nature of the Scatchard plot of epidermal growth factor (EGF) binding on EGF receptor (EGFR) has been a controversial issue for more than a decade. Past efforts to mechanistically simulate the concave up nature of the Scatchard plot of EGF binding have shown that negative cooperativity in EGF binding on an EGFR dimer or inclusion of some external site or binding event can describe this behavior. However, herein we show that heterogeneity in the density of EGFR due to localization in certain regions of the plasma membrane, which has been experimentally reported, can also lead to concave up shape of the Scatchard plot of the EGF binding on EGFR.
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| http://dx.doi.org/10.1016/j.febslet.2005.04.059 | DOI Listing |
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