AI Article Synopsis
- Genistein is a natural isoflavone that enhances cell death and apoptosis from DNA-damaging agents, like camptothecins, in cancer cell lines (HeLa and OAW-42) and normal fibroblasts (L929).
- The study used the SRB method in 96-well plates for analysis, showing that genistein effectively boosts the anti-proliferative effects of camptothecins by disrupting the G2/M cell cycle checkpoint.
- In HeLa cells, genistein reduces CDK1 phosphorylation following irinotecan treatment, suggesting that it could be a strategic way to increase the effectiveness of DNA-damaging treatments.
Article Abstract
Genistein, a natural isoflavone product has been shown to induce cellular death and increase the apoptotic cell death induced by several DNA-damaging stimuli. We have explored the combined effect of genistein and camptothecins against three cell lines, HeLa (cervical cancer), OAW-42 (ovarian cancer) and L929 (normal fibroblasts). Combined effect was estimated in 96-well plates using the SRB method and median-effect analysis. Addition of genistein synergistically increased the antiproliferative affect of camptothecins, inhibiting the camptothecin-induced G2/M arrest and increasing the apoptotic cell population. In HeLa cells, genistein inhibited CDK1 phosphorylation after irinotecan treatment. Thus, abrogation of the G2/M checkpoint control by genistein may be a useful maneuver to increase cytotoxicity of agents that damage DNA and inhibit cell-cycle progression in the G2/M boundary.
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| http://dx.doi.org/10.1016/j.canlet.2005.03.022 | DOI Listing |
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