Microsporidia are obligate intracellular parasites. Microsporidian spores infect a wide variety of hosts, including humans. The spores may be found in infected hosts' urine and feces, thus waterborne transmission is possible. This study details method development for the detection of microsporidia in tap water. In this study, filtration, centrifugation, purification, and detection parameters were optimized for the detection of microsporidia. The Pall-Gelman Envirocheck sampling capsule (Pall Gelman, Ann Arbor, MI) was chosen as the filter element. Optimal centrifugal force for spore recovery was 1500 x g. Additionally, it was determined that eluting microsporidia spores in a detergent elution buffer solution had a detrimental effect on spore recovery. A direct examination of the concentrate resulted in a greater recovery with less variability than subjecting the sample concentrate to a Percoll-sucrose density gradient purification step. The staining method employed for the detection spores was Calcofluor white (Sigma, St. Louis, MO). Percent recoveries for 10 L tap water samples (n = 5) using the Envirocheck sampling capsule without a density gradient purification step were 26.1+/-13.4 compared to 25+/-13.8 for samples subjected to a density gradient purification step.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1081/ese-200056105 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
TRACE ORGANIC COMPOUNDS AND PHOTOSENSITIZING ACTIVITY IN SALVADORAN SURFACE AND TAP WATER SOURCES: A FIRST LOOK.
Environ Pollut
January 2025
University of Arizona, Chemical and Environmental Engineering Department.
Despite their potential risks to human health and the environment at ng/L to μg/L concentrations, there has been relatively little effort to measure trace organic compounds (TOrCs) in surface waters of Central America. The concentrations of eighteen TOrCs detected at eleven surface water sites in the Lempa River basin of El Salvador and four sources of drinking water for the cities of San Salvador, Antiguo Cuscatlán, Soyapango, and Santa Tecla are reported here. All samples were analyzed via liquid chromatography with tandem mass spectrometry (LC-MS/MS).
View Article and Find Full Text PDFBenzotrithiophene-based covalent organic frameworks for sensitive fluorescence detection and efficient removal of Ag from drinking water.
Talanta
December 2024
Department of Food Science and Engineering, College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen 518060, China; Shenzhen Key Laboratory of Food Macromolecules Science and Processing, Shenzhen University, Shenzhen 518060, China. Electronic address:
The simultaneous detection and removal of Ag from drinking water was crucial for preventing human health, while it was also extremely challenging due to bifunctional materials that combine both Ag adsorption and detection functions rarely being explored. In this study, a benzotrithiophene-based covalent organic framework (TAPA-BTT) was synthesized and applied to detect and remove Ag. TAPA-BTT exhibited high crystallinity, a large specific surface area, and good thermal stability.
View Article and Find Full Text PDFSpatial assessment of drinking water flavor in China: Revealing regional disparities and underlying drivers.
Water Res
December 2024
The Quzhou Affiliated Hospital of Wenzhou Medical University, Quzhou People's Hospital, Quzhou 324000, China.
Drinking water flavor, a critical water quality metric, exhibits substantial regional variations across China, influenced by local geology and chemistry. Despite growing consumer concerns about water flavor, a spatial assessment of the determinants of water flavor in China has been notably lacking. This study bridges this gap by conducting a spatially comprehensive analysis of 78 tap water samples throughout China.
View Article and Find Full Text PDFFluorescent "on-off-on" sensor based on N, P co-doped carbon nanoparticles for specific detection of Fe and ascorbic acid.
J Food Drug Anal
December 2024
Zhenjiang College, Zhenjiang, 212000, PR China.
Ascorbic acid (AA) is used as a food additive for its antibacterial and antioxidant properties. However, excessive intake of AA is harmful to humans. Therefore, the detection of Fe and AA is generally recognized to be meaningful.
View Article and Find Full Text PDFA quality by design HPLC method for cephalosporin analysis in pharmaceuticals and water samples with environmental impact assessment.
Sci Rep
January 2025
Egyptian Drug Authority (EDA), Giza, 35521, Egypt.
Article Synopsis
- The study developed a new HPLC method for analyzing four cephalosporin antibiotics in formulations and water, focusing on sustainability.
- A Box-Behnken experimental design was used to optimize key parameters like mobile phase composition and flow rate, resulting in effective separation in under 6 minutes.
- The method was validated for accuracy, precision, and environmental impact, scoring 0.75 on the AGREE scale, indicating it is a greener choice for routine testing.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!