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Semiautomated Production of Cell-Free Biosensors.
ACS Synth Biol
March 2025
Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208, United States.
Cell-free synthetic biology biosensors have potential as effective diagnostic technologies for the detection of chemical compounds, such as toxins and human health biomarkers. They have several advantages over conventional laboratory-based diagnostic approaches, including the ability to be assembled, freeze-dried, distributed, and then used at the point of need. This makes them an attractive platform for cheap and rapid chemical detection across the globe.
View Article and Find Full Text PDFComparing manual and automated methods for calculating speaking rate in Parkinson's disease.
JASA Express Lett
March 2025
Department of Communicative Disorders and Sciences, University at Buffalo, Buffalo, New York 14214, USA.
This study compared manual and automated methods for calculating speaking rate in recorded samples from individuals with Parkinson's disease. The manual procedure involved trained researchers measuring speaking rate through manual counting and acoustic analysis of speech units and pauses, while the automated method utilized a custom praat script developed by de Jong and Wempe [(2009). Behav.
View Article and Find Full Text PDFTraining healthcare professionals in hypnosis-derived communication to mitigate procedural pain in children.
Sci Rep
March 2025
Department of Anesthesiology, Université de Montréal, Montreal, Qc, Canada.
How professionals communicate during medical procedures may have a significant impact on children and adolescents' pain. Rel@x is a manualized training program designed to develop hypnosis-derived communication skills to mitigate childhood pain and distress. The study aimed to evaluate if this training was associated with an improvement and maintenance in communication skills over time, and measure associations between changes and participants' characteristics.
View Article and Find Full Text PDFSignificantly increased load of hereditary cancer-linked germline variants in infertile men.
Hum Reprod Open
February 2025
Chair of Human Genetics, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia.
Study Question: What is the load and profile of hereditary cancer-linked germline variants in infertile compared to fertile men?
Summary Answer: This study showed almost 5-fold enrichment of disease-causing findings in hereditary cancer genes in infertile compared to fertile men (6.9% vs 1.5%, =2.
Recent advances in spatial transcriptomics (ST) technologies have transformed our ability to profile gene expression while retaining the crucial spatial context within tissues. However, existing ST platforms suffer from high costs, long turnaround times, low resolution, limited gene coverage, and small tissue capture areas, which hinder their broad applications. Here we present iSCALE, a method that predicts super-resolution gene expression and automatically annotates cellular-level tissue architecture for large-sized tissues that exceed the capture areas of standard ST platforms.
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