AI Article Synopsis
- The study used the CAP-Trapper method to create human full-length-enriched cDNA libraries, leading to the identification of 342 potential new human genes.
- About 23,000 clones from various tissues and developmental stages were sequenced, revealing 5,300 unique cDNAs, with 40% extending previously known gene annotations and 662 being splice variants.
- Gene expression analysis indicated that 260 out of the 342 unknown clones were expressed in various cell lines or tissues, suggesting they could be novel genes, although only a small number are likely to code for proteins.
Article Abstract
In this work we describe the process that, starting with the production of human full-length-enriched cDNA libraries using the CAP-Trapper method, led us to the discovery of 342 putative new human genes. Twenty-three thousand full-length-enriched clones, obtained from various cell lines and tissues in different developmental stages, were 5'-end sequenced, allowing the identification of a pool of 5300 unique cDNAs. By comparing these sequences to various human and vertebrate nucleotide databases we found that about 40% of our clones extended previously annotated 5' ends, 662 clones were likely to represent splice variants of known genes, and finally 342 clones remained unknown, with no or poor functional annotation. cDNA-microarray gene expression analysis showed that 260 of 342 unknown clones are expressed in at least one cell line and/or tissue. Further analysis of their sequences and the corresponding genomic locations allowed us to conclude that most of them represent potential novel genes, with only a small fraction having protein-coding potential.
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| http://dx.doi.org/10.1016/j.ygeno.2005.02.009 | DOI Listing |
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