The anomalous false-positive results often found in the primed lymphocyte typing (PLT) test seem to be partially due to the release of blastogenic factor (BF) from the stimulating cell, resulting in back stimulation of the primed lymphocyte. These anomalous responses can be greatly reduced by treating the stimulator cell with high doses of irradiation or with puromycin.
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Toward a molecular understanding of adaptive immunity: a chronology, part I.
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The adaptive immune system has been the core of immunology for the past century, as immunologists have been primarily focused on understanding the basis for adaptive immunity for the better part of this time. Immunological thought has undergone an evolution with regard to our understanding as the complexity of the cells and the molecules of the system became elucidated. The original immunologists performed their experiments with whole animals (or humans), and for the most part they were focused on observing what happens when a foreign substance is introduced into the body.
View Article and Find Full Text PDFMeeting report of the International Symposium of Molecular Cell Biology of Macrophages '94.
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Department of Pharmacology, Kanazawa University, Japan.
The International Symposium of Molecular Cell Biology of Macrophages '94 was held on February 10-11, 1994 at Kanazawa University, Kanazawa, Japan. Fifteen speakers including 4 speakers from Europe and the United States were invited to this symposium. In spite of unusual heavy snow and chilly weather, 150 macrophage scientists gathered and had hot discussions.
View Article and Find Full Text PDF[Basis of cytokines--history and present status].
Rinsho Byori
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1st Department of Internal Medicine, Kyushu University, Fukuoka.
Cytokines are high molecular-weight substances which actively promote homeostasis and defend from exogenous agents. In 1965, Kasakura, et al, and Gordon, et al independently published articles describing an unknown substance (blastogenic factor) which was produced in the cultured media of lymphocytes and stimulated lymphocytic proliferation. In those days, since almost all immunologists believed that only antigens stimulated lymphocytic proliferation, the idea that this soluble factor actually had a positive role in lymphocytic proliferation was viewed suspiciously.
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Institute of Virology, University La Sapienza, Rome, Italy.
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