The purpose of this study was to investigate how aragonite (calcium carbonate) whiskers influenced the strengthening and carbonating of alpha-tricalcium phosphate (alpha-TCP) based calcium phosphate cement. Aragonite whiskers of 0.95 microm width with an aspect ratio of 6.6 were prepared. The cement powder, alpha-TCP containing 0-50 mass% aragonite whisker, was mixed with 0.6 mol/L NaH2PO4 solution and incubated at 37 degrees C and 100% relative humidity. Diametral tensile strength (DTS) value increased significantly when appropriate amount of aragonaite whiskers was added. For example, DTS value of set cement containing 20 mass% aragonite whisker was 5.8 +/- 0.5 MPa, whereas DTS value of set cement containing no whiskers was 1.3 +/- 0.2 MPa after 1-week incubation. SEM observation revealed that the shape of the whiskers and the densification of the structure could have contributed to the strengthening of the set cement. Moreover, FTIR spectra implied that a bone-like carbonated apatite was precipitated in the cement. The results obtained in the present study revealed that the shape as well as any slight dissolution of aragonite whiskers could contribute to improving the properties of a-TCP based calcium phosphate cement.
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http://dx.doi.org/10.4012/dmj.24.104 | DOI Listing |
Bio Protoc
January 2025
Department of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, Taiwan.
Primary neuronal culture and transient transfection offer a pair of crucial tools for neuroscience research, providing a controlled environment to study the behavior, function, and interactions of neurons in vitro. These cultures can be used to investigate fundamental aspects of neuronal development and plasticity, as well as disease mechanisms. There are numerous methods of transient transfection, such as electroporation, calcium phosphate precipitation, or cationic lipid transfection.
View Article and Find Full Text PDFCureus
December 2024
Orthopaedics and Traumatology, Unidade Local de Saúde de Viseu Dão-Lafões, Viseu, PRT.
Reverse Hill-Sachs lesions (RHSL) are common complications associated with posterior shoulder dislocations and represent a significant challenge for preserving joint stability and function. If untreated, these compression fractures of the anteromedial humeral head can compromise the integrity of the joint, predisposing patients to recurrent instability and arthropathy. While various treatment modalities exist, achieving an anatomic reduction of the defect while preserving the articular cartilage remains a desirable outcome, particularly in acute settings.
View Article and Find Full Text PDFJ Nanobiotechnology
January 2025
Orthodontic Department, Nanjing Stomatological Hospital, Affiliated hospital of Medical School, Institute of Stomatology, Nanjing University, No. 30 Zhongyang Road, Nanjing, Jiangsu, China.
BMC Biotechnol
January 2025
Department of Oral Science and Translation Research, College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, FL, 33314, USA.
Background: Tissue engineering for bone regeneration aims to heal severe bone injuries. This study aimed to prepare and assess the early osteogenic differentiation effects of a gelatin/calcium phosphate- Punica granatum nanocomposite scaffold on stem cells from human exfoliated deciduous (SHED) and human dental pulp stem cells (HDPSCs).
Methods: The electrospinning method was used to prepare a gelatin/calcium phosphate nanocomposite scaffold containing pomegranate (Punica granatum) extract.
Acta Bioeng Biomech
September 2024
Department of Biochemistry and Biotechnology, Medical University of Lublin, Lublin, Poland.
: The synthesis of fluoridated apatite consists of several stages, among which the heat treatment has a significant impact on the physical and chemical properties. The present study aims to elucidate the influence of two different sintering methods on fluoride-substituted apatite properties. : For this purpose, a two F-substituted apatites were produced by heat treatment in different ways called "rapid sintering" and "slow sintering".
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