We report here on an integrated microfabricated device dedicated to the preparation of biological samples prior to their on-line analysis by electrospray ionization-mass spectrometry (ESI-MS). This microfluidic device is fabricated using the negative photoresist SU-8 by microtechnology techniques. The device includes a chromatographic module plus an ESI interface for MS. The chromatographic module is dedicated to sample purification and is based on a polymer monolithic phase which includes hydrophobic moieties. The ESI interface is integrated onto the chip and is based on a capillary slot. We present here the integration of these different modules onto a single system that is fabricated via a SU-8-based microtechnology route. We present also their testing for the purification of peptide samples. This started with a partial integration step with the combination of at least two of the modules (microsystem + monolith; microsystem + nib) and their test before the fabrication and testing of fully integrated microsystems.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chroma.2004.12.037DOI Listing

Publication Analysis

Top Keywords

integrated microfabricated
8
chromatographic module
8
esi interface
8
integrated
4
microfabricated systems
4
systems including
4
including purification
4
purification module
4
module on-chip
4
on-chip nano
4

Similar Publications

Cell-matrix interactions, mediated by cellular force and matrix remodeling, result in dynamic reciprocity that drives numerous biological processes and disease progression. Currently, there is no available method for directly quantifying cell traction force and matrix remodeling in three-dimensional matrices as a function of time. To address this long-standing need, we developed a high-resolution microfabricated device that enables longitudinal measurement of cell force, matrix stiffness and the application of mechanical stimulation (tension or compression) to cells.

View Article and Find Full Text PDF

In this work, an integrated microfluidic microwave array sensor is proposed for the enrichment and detection of mixed biological solution. In individuals with urinary tract infections or intestinal health issues, the levels of white blood cells (WBCs) and () in urine or intestinal extracts can be significantly elevated compared to normal. The proposed integrated chip, characterized by its low cost, simplicity of operation, fast response, and high accuracy, is designed to detect a mixed solution of WBCs and .

View Article and Find Full Text PDF

Microspectrometer-Enabled Real-Time Concentration Monitoring in the Microfluidic Protein Enrichment Chip.

Biosensors (Basel)

December 2024

Department of Mechanical Engineering and Advanced Institute of Manufacturing for High-Tech Innovations, National Chung Cheng University, Chia-Yi 62102, Taiwan.

This study presents a novel microspectrometer-integrated microfluidic system for real-time protein concentration monitoring. The device employs electrokinetic principles for efficient protein preconcentration in a PDMS and Nafion film channel. Using FITC-labeled BSA as a model protein, the system demonstrated a linear correlation between protein concentration and absorbance at 491 nm.

View Article and Find Full Text PDF

Implantable multifunctional probes have transformed neuroscience research, offering access to multifaceted brain activity that was previously unattainable. Typically, simultaneous access to both optical and electrical signals requires separate probes, while their integration into a single device can result in the emergence of photogenerated electrical artifacts, affecting the quality of high-frequency neural recordings. Among the nontrivial strategies aimed at the realization of an implantable multifunctional interface, the integration of optical and electrical capabilities on a single, minimally invasive, tapered optical fiber probe has been recently demonstrated using fibertrodes.

View Article and Find Full Text PDF

Microfluidics Based Particle and Droplet Generation for Gene and Drug Delivery Approaches.

J Biomed Mater Res B Appl Biomater

February 2025

Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Ege University, Izmir, Turkey.

Microfluidics-based droplets have emerged as a powerful technology for biomedical research, offering precise control over droplet size and structure, optimal mixing of solutions, and prevention of cross-contamination. It is a major branch of microfluidic technology with applications in diagnostic testing, imaging, separation, and gene amplification. This review discusses the different aspects of microfluidic devices, droplet generation techniques, droplet types, and the production of micro/nano particles, along with their advantages and limitations.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!