AI Article Synopsis
- Neutrophils are crucial for killing bacteria after infections, and this study investigates how ultra-high molecular weight polyethylene (UHMWP) particles might disrupt their function.
- Experiments showed a significant decrease in neutrophil bacterial killing as the concentration of UHMWP particles increased, particularly with 10^7 UHMWP/ml and smaller particle sizes (0.1-5 microm).
- The findings suggest that UHMWP particles could impair neutrophil activity, potentially increasing the risk of bacterial infections in patients with loose implants.
Article Abstract
Following infection, bacterial killing by polymorphonuclear leukocytes (neutrophils) is the main host defense against bacteria. Our hypothesis is that particles of ultra-high molecular weight polyethylene (UHMWP) may impair local neutrophil function and consequently reduce neutrophil bacterial killing. To determine how the in vitro phagocytic-bactericidal activity of neutrophils was affected by exposure to wear particles, tests were run comparing the effects of different particle composition, and different concentrations and sizes of UHMWP particles. There was a significant correlation between the number of particles and the decrease in neutrophil bactericidal activity (p<0.01), and the greatest effect was obtained with a concentration of 10(7) UHMWP/ml. There was a significant decrease in neutrophil bactericidal activity by incubation with particles of 0.1-5 microm (p<0.01), but not with larger size. The results suggest that neutrophil functional defects triggered by the presence of UHMWP particles may potentially contribute to the susceptibility of loose implants to bacterial infections.
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| http://dx.doi.org/10.1016/j.biomaterials.2004.08.034 | DOI Listing |
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