Objective: To establish a method of determinating content of bilirubin in Niuhuang Compound Suppository by HPLC.
Method: C18 column was used. The mobile phase was consisted of dimethylsulfoxide: acetonitrile: 0.5% acetic ammonium (1:1.4:1). Detector wavelength was 456 nm. The extraction solution for bilirubin was consisted of dlimethylsulfoxide: acetonitrile = 9:4.
Results: The linearity was obtained over the range of 0.107 - 2.14 microg. The mean recovery was 101.0%, RSD = 0.518% (n = 5).
Conclusion: The method was sensitive, simple and accurate.
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