AI Article Synopsis
- The study focused on optimizing the preparation of anemonin from Ranunculus japonicus Thunb. using various experimental methods.
- The ideal extraction conditions were determined to be 15% NaCl, extracting four times, and drying at 60 degrees Celsius, resulting in a 99% purity level.
- The preparation techniques proved to be effective and easy to implement, making them suitable for further use.
Article Abstract
Objective: To study the preparation of anemonin from Ranunculus japonicus Thunb.
Methods: The best conditions of preparation for anemonin were optimized with monosyllabic experiments, such as using the purity of anemonin as a index for best selection of dying tempreture and the extraction ratio as another index for the concentration of NaCl and extracting times. The structures were clucidated on the basis of UV, IR, 1HNMR, 13CNMR, 1H-1H COSY, HMQC and HMQC spectra and chemical reactions and the purity is measured by HPLC.
Results: The best conditions for the extraction techniques of anemonin were 15% NaCl, four extracting times, 60 degrees C (0.08 Mpa) for drying. The purity of anemonin was 99% and yield rate was 0. 72 per thousand. Its quality was controlled.
Conclusion: The preparation techniques are feasible and convenient.
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