Objective: To investigate immuno-modulating activity change after mycelial polysaccharide from Cordyceps gunnii (MPCG) was depolymerized by ultrasonication.
Methods: The effects of MPCG and its depolymerizing mixture on mice spleen lymphocytes proliferation, mice peritoneal macrophage (PMphi) phagocytosis and cytotoxin T lymphocytes (CTL) activity were studied by MTT method, neutral red colorimetry, respectively.
Results: In vitro, MPCG depolymerized by ultrasonication could more significantly inhibite spleen lymphocytes proliferation, PMphi phagocytosis of neutral red and CTL activity in mice than MPCG, in particular at doses of 10 microg/ml and 100 microg/ml. Whereas, four kinds of depolymerized mixture depolymerized for 1h to 2h and 4h to 8h by ultrasonication, respectively, had not evident differences at three various doses.
Conclusion: MPCG was suitable for depolymerizing for 1h to 2h and its depolymerized mixture had stronger immuno-modulating activity than MPCG.
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