To investigate the effect of recombinant plasmid of Helicobacter pylori ureB gene on gastric epithelial cell. The full length sequence of ureB gene from NCTC11637 was amplified by PCR. The recombinant plasmid was constructed by cloning the open reading frame (ORF) of ureB into the eukaryotic expression vector pcDNA3.1, and was transfected SGC-7901 cells, then the clones resisting Hygromycin were screened. mRNA expression of ureB of transfected cells was detected by RT-PCR. The effect of recombinant plasmid of Hp ureB gene on cell phenotype was observed by fluorescence strain, on proliferation by MTT, on apoptosis and cell cycles by flow cytometry, respectively. The positive clones of ureB (SureB) appeared cell membrane budding and cell shrinkage. MTT assay showed there was no statistic significance between the SureB and SpcDNA3.1 which were transfected only by pcDNA3.1 (P > 0.05), suggesting that the growth of SureB were not inhibited. The apoptosis rate of SureB was higher than that of SpcDNA3.1 (P = 0.007). Analysis for cell cycle showed that in SureB cells the proportion of S phase increased, the proportion of both G2/M and G0/G1 phase decreased. Positive transfection of ureB gene into SGC-7901 can change cell phenotype and induce cell apoptosis.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Bacterial biomineralization of heavy metals and its influencing factors for metal bioremediation.
J Environ Manage
January 2025
Laboratory of Environmental Microbiology and Ecology (LEnME), Department of Life Science, National Institute of Technology, Rourkela, 769 008, Odisha, India. Electronic address:
Increasing industrial pollution and certain hazardous agricultural practices have led to the discharge of heavy toxic metals into the environment. Among different bioremediation techniques, biomineralization is the synthesis of biomineral crystals extracellularly or intracellularly. Several bacteria, such as Bacillus cereus, Pseudomonas stutzeri, Bacillus subtilis, and Lactobacillus sphaericus have been found to induce heavy metal precipitation and mineralization for bioremediation.
View Article and Find Full Text PDF[Impact of Organic Amendment on the Bacterial Community and Rice Yield in Paddy Soil].
Huan Jing Ke Xue
January 2025
State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
In this investigation, the influence of organic amendment on the structural and functional dynamics of soil microbial communities and its effect on rice productivity were examined. Five fertilization treatments from a 40-year field experiment were selected： no fertilizer （CK）, inorganic NPK fertilizer （NPK）, inorganic NPK combined with green manure （NG）, inorganic NPK combined with green manure and pig manure （NGM）, and inorganic NPK combined with green manure and rice straw （NGS）. The findings revealed that the organic amendment enhanced the soil organic carbon （SOC）, total nitrogen （TN）, and total phosphorus （TP） levels, alongside an increase in rice yield； notably, the most significant improvements were observed with the NGM treatment.
View Article and Find Full Text PDFPreliminary investigation of the anti- activity of Triphala.
Front Pharmacol
November 2024
School of Medicine and Pharmacy, Ocean University of China, Qingdao, Shandong, China.
Background: Triphala, is a composite of three individual botanical drugs: , , and . It exhibits properties such as heatclearing, anti-inflammatory, anti-fatigue, antioxidant, and antibacterial effects,making it extensively utilized in India and Tibet. It has been found to exhibitinhibitory effects on (); however, further comprehensive research is still needed to elucidate its specific antibacterial mechanism.
View Article and Find Full Text PDFComparative genomics unravels a rich set of biosynthetic gene clusters with distinct evolutionary trajectories across fungal species (Termitomyces) farmed by termites.
Commun Biol
October 2024
Section for Ecology and Evolution, Department of Biology, University of Copenhagen, Universitetsparken 15, 2100, Copenhagen, Denmark.
The use of compounds produced by hosts or symbionts for defence against antagonists has been identified in many organisms, including in fungus-farming termites (Macrotermitinae). The obligate mutualistic fungus Termitomyces plays a pivotal role in plant biomass decomposition and as the primary food source for these termites. Despite the isolation of various specialized metabolites from different Termitomyces species, our grasp of their natural product repertoire remains incomplete.
View Article and Find Full Text PDFCharacterization of urease active calcite-producing strain YX-3 combined with the whole genome.
Environ Res
December 2024
College of Life Science, Northwest University, 229 Tai bai North Rd, Xi'an, Shaanxi, 710069, China; Shaanxi Provincial Key Laboratory of Biotechnology, Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Science, Northwest University, Xi'an, Shaanxi, 710069, China. Electronic address:
Article Synopsis
- Urease in microorganisms is crucial for calcite precipitation, but there's limited genomic data on urease-producing strains, highlighting a need for deeper research into its activity regulation by nickel ligands.
- The study focused on a newly isolated strain YX-3, revealing the presence of urease gene clusters and demonstrating that nickel ions significantly enhance the expression of the ureC gene.
- Mutations in specific residues (His267, His294, Gly325) were shown to decrease urease activity and calcium carbonate precipitation, indicating their essential role in urease functionality and providing insights for future research on urease regulation.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!