AI Article Synopsis
- A large-scale strain screening was performed to create a bioprocess for producing 6-hydroxynicotinic acid.
- A soil-derived strain named NA-1 was found to effectively convert nicotinic acid into 6-hydroxynicotinic acid, identified through techniques like HPLC, MS, and 1H-NMR.
- Morphological, physiological, and biochemical analyses confirmed that NA-1 closely resembles Pseudomonas putida, with a 99% sequence identity based on 16S rDNA analysis.
Article Abstract
Strain screening was conducted on a large scale in order to develop a biotransforming process for 6-hydroxynicotinic acid production. A strain named as NA-1 was isolated from soil which could transform nicotinic acid into a new product showed by HPLC. The new product was identified as 6-hydroxynicotinic acid by MS and 1H-NMR. The morphology, physiological and biochemical characteristics of strain NA-1 were studied and showed that characteristics of strain NA-1 were essentially consistent with Pseudomonas putida. The analysis of 16S rDNA sequence from strain NA-1 suggested that strain NA-1 was clustered together with P. putida in phylogenetic tree and the sequence identity between stain NA-1 and Pseudomonas putida was 99%. So strain NA-1 was identified to P. putida.
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