AI Article Synopsis
- Environmental DNA was extracted from sediment in the Parece Vela Basin at a depth of 5010 m in the Pacific Ocean.
- A 16S rRNA gene library was created, revealing 17 distinct phylotypes primarily consisting of gamma-Proteobacteria, alpha-Proteobacteria, and marine uncultured bacteria.
- 62% of the sequences were closely related to known bacteria in the Halomonas, Alcanivorax, Pseudomonas, Acinetobacter, and Pseudoalteromonas genera, while others suggested potential novel taxa.
Article Abstract
The environmental DNA was directly extracted from the sediment in Parece Vela Basin, Pacific Ocean, at a depth of 5010 m. Bacterial 16S rRNA gene library of 32 clones was generated using bacterial universal primers and 16S rDNA sequences were analyzed phylogenetically. 17 phylotypes were obtained. The library was dominated by gamma-Proteobacteria, alpha-Proteobacteria and marine uncultured bacteria. Sixty-two percent of the cloned sequences was highly related to the known bacteria in the genus Halomonas, Alcanivorax, Pseudomonas, Acinetobacter, Pseudoalteromonas (> 96% sequence similarity), while some of the cloned sequences showed less affiliation with known taxa (< 94% sequence similarity) and may represent novel taxa.
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Article Synopsis
- Environmental DNA was extracted from sediment in the Parece Vela Basin at a depth of 5010 m in the Pacific Ocean.
- A 16S rRNA gene library was created, revealing 17 distinct phylotypes primarily consisting of gamma-Proteobacteria, alpha-Proteobacteria, and marine uncultured bacteria.
- 62% of the sequences were closely related to known bacteria in the Halomonas, Alcanivorax, Pseudomonas, Acinetobacter, and Pseudoalteromonas genera, while others suggested potential novel taxa.
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