Objective: To study the nuclear translocation of extracellular signal regulated kinase (ERK) and expression of c-fos mRNA under stimulation of AngII as well as the influence on the nuclear translocation of ERK with different interferences in neonatal cultured cardiomyocytes.
Methods: ERK in the cytoplasm or nucleus was observed by immunocytochemistry using specific antibody and the expression of c-fos was evaluated with RT-PCR technique.
Results: AngII (10(-6) mol/L) had the effect in promoting activation of ERK and then appeared in nucleus rapidly. The translocation process of ERK induced by AngII was blocked distinctly by Valsartan (10(-5) mol/L) and PD98059 (5 x 10(-5) mol/L), but not by CGP42112A (10(-5) mol/L). It was also found that Valsartan and PD98059 could inhibit the expression of c-fos in that process.
Conclusion: The nuclear translocation of ERK might be a precondition for the inducement of c-fos expression.
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