Objective: To investigate the methylation status of LRP15 gene in acute leukemia (AL) and its role in tumorigenesis.
Methods: 73 cases of AL and 9 healthy subjects as well as COS7, K562 and HL60 cell lines were studied with methylation specific PCR (MSP).
Results: LRP15 was not detected in all the samples. No LRP15 methylation was detected in COS7, but LRP15 was methylated in K562 and HL60. In nearly all of the French-American-British leukemia subtypes, we found that the frequency of LRP15 methylation was 71.2% (52/73) of AL and none in the 9 healthy subjects. The difference in mean methylation for LRP15 between these two kinds of samples is statistically significant (P < 0.05). Hypermethylation of the LRP15 gene was found in 57.1% (16/28) of the newly diagnosed AL and 83.3% of the relapsed AL respectively; this is also statistically significant (P < 0.05).
Conclusion: LRP15 methylation change is a common abnormality in leukemia and LRP15 is postulated to be a tumor suppressor gene.
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The novel gene LRP15 is regulated by DNA methylation and confers increased efficiency of DNA repair of ultraviolet-induced DNA damage.
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LRP15 is a novel gene cloned from lymphocytic cells, and its function is still unknown. Bioinformatic data showed that LRP15 might be regulated by DNA methylation and had an important role in DNA repair. In this study, we investigate whether the expression of LRP15 is regulated by DNA methylation, and whether overexpression of LRP15 increases efficiency of DNA repair of UV-induced DNA damage in HeLa cells.
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Chin Med Sci J
September 2007
Department of Hematology, Chinese People's Liberation Army General Hospital, Beijing 100853.
Objective: To investigate the methylation status of LRP15 gene in acute leukemia (AL) patients and its role in the tumorigenesis.
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Zhongguo Shi Yan Xue Ye Xue Za Zhi
February 2007
Department of Hematology, The General Hospital of PLA, Beijing 100853, China.
To investigate the relationship between LRP15 gene promoter region methylation and its gene expression in acute leukemia patients, the status of LRP15 gene promoter region methylation was detected by MS-PCR and the gene expression was detected by RT-PCR in bone marrow samples from leukemia patients. The results indicated that the LRP15 gene expression was 47.6% in complete remission (CR) patients and 16.
View Article and Find Full Text PDF[Analysis of the methylation in the promoter of LRP15 gene and its expression].
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To study the methylation in the promoter of LRP15 gene and its relationship with gene expression and to explore the possible mechanism of regulating LRP15 gene methylation, the methylation in the promoter of LRP15 gene in K562 cell line was detected by MS-PCR. Then K562 was exposed to 5-aza-2'-deoxycytidine (CdR) and trichostatin (TSA), to determine whether the silencing of LRP15 gene by de novo methylation could be reversed. As a result, it was confirmed by MS-PCR that the promoter of LRP15 was hypermathylated in K562 cell line, and lost its transcription activity.
View Article and Find Full Text PDF[The methylation pattern of LRP15 gene in acute leukemia].
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Department of Hematology, PLA General Hospital, Beijing 100853, China.
Objective: To investigate the methylation status of LRP15 gene in acute leukemia (AL) and its role in tumorigenesis.
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