AI Article Synopsis
- * Transgenic plants that over-expressed PAP1 cDNA or contained a PAP1 repressor showed decreased PA levels, despite increased soluble anthocyanins in those seeds.
- * The study indicates that PA is crucial for radical scavenging activity in seeds and suggests potential for engineering transcription factors to enhance beneficial compounds in plant seeds.
Article Abstract
The proanthocyanidin (PA) content was increased in seeds of pap1-D mutant of Arabidopsis thaliana, in which the expression of endogenous PAP1 gene encoding a Myb-like transcription factor was induced by activation-tagging with enhancer sequences derived from cauliflower mosaic virus 35S promoter. In contrast, the PA contents decreased in seeds of transgenic plants transformed with a PAP1 cDNA or with a PAP1 chimeric repressor, although the amount of soluble anthocyanins increased in seeds of transgenic plants over-expressing PAP1 cDNA. The enhanced radical scavenging activity was observed only in the seed extracts of pap1-D mutant, indicating that PAs are primarily responsible for radical scavenging activity in seeds. The present study suggests the feasibility of engineering a transcription factor of flavonoid biosynthesis for health beneficial plant seeds.
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| http://dx.doi.org/10.1007/s10529-005-0683-7 | DOI Listing |
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