AI Article Synopsis
- The study focuses on using single-molecule atomic force microscopy (AFM) to image DNA molecules that self-assemble on a hydrophobic surface (HOPG).
- It observes that the DNA interactions with the surface lead to aggregation and complex molecular interactions, emphasizing the importance of controlling electrode potential for accurate imaging.
- Applying a +300 mV potential improves DNA stability on the electrode by strengthening electrostatic interactions between the positively charged surface and the negatively charged DNA.
Article Abstract
Single-molecule AFM imaging of single-stranded and double-stranded DNA molecules self-assembled from solution onto a HOPG electrode surface is reported. The interaction of DNA with the hydrophobic surface induced DNA aggregation, overlapping, intra- and intermolecular interactions. Controlling the electrode potential and using the phase images as a control method, to confirm the correct topographical characterization, offers the possibility to enlarge the capability of AFM imaging of DNA immobilized onto conducting substrates, such as HOPG. The application of a potential of +300 mV (versus AgQRE) to the HOPG enhanced the robustness and stability of the adsorbed DNA molecules, increasing the electrostatic interaction between the positively charged electrode surface and the negatively charged DNA sugar-phosphate backbone.
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| http://dx.doi.org/10.1016/j.bioelechem.2004.05.009 | DOI Listing |
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