Nonhemolytic antibody-induced loss of erythrocyte surface antigen.

Blood

Transfusion Medicine Program, Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Woodruff Memorial Bldg Ste 7301, 101 Woodruff Cir, Atlanta, GA 30322, USA.

Published: August 2005

AI Article Synopsis

Article Abstract

Transfusion of red blood cells (RBCs) into patients with anti-donor RBC antibodies (crossmatch-incompatible transfusion) can result in lethal antibody-mediated hemolysis. Less well appreciated is the ability of anti-RBC antibodies to specifically remove their target antigen from donor RBCs without compromising cell survival or adversely affecting the transfusion recipient. In an effort to elucidate the mechanistic details of this process, we describe the first animal model of nonhemolytic antibody-induced RBC antigen loss. RBCs from transgenic mHEL mice express surface hen egg lysozyme (HEL) as a transmembrane protein. Transfusion of mHEL RBCs into mice immunized with HEL results in selective loss of HEL antigen from donor RBCs without affecting other blood group antigens or reducing the circulatory life span of the transfused RBCs. While this process does not require the presence of a spleen, it requires both anti-RBC immunoglobulin G (IgG) antibodies and the FcgammaIII receptor. These studies provide mechanistic insight into the phenomenon of antigen loss during incompatible transfusion in humans.

Download full-text PDF

Source
http://dx.doi.org/10.1182/blood-2005-03-1040DOI Listing

Publication Analysis

Top Keywords

nonhemolytic antibody-induced
8
antigen donor
8
donor rbcs
8
antigen loss
8
rbcs
6
antigen
5
transfusion
5
loss
4
antibody-induced loss
4
loss erythrocyte
4

Similar Publications

Background: In antibody-mediated nonhemolytic transfusion reactions, transfusion-related acute lung injury (TRALI) tends to occur typically within 2 hours after a blood transfusion. White cell antibodies or immune complexes have been frequently shown to be associated with the syndrome, although the mechanisms by which they induce TRALI are poorly understood. The aim of this study was to characterize soluble mediators that are released from cells at an early stage after immune stimulation.

View Article and Find Full Text PDF

Nonhemolytic antibody-induced loss of erythrocyte surface antigen.

Blood

August 2005

Transfusion Medicine Program, Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Woodruff Memorial Bldg Ste 7301, 101 Woodruff Cir, Atlanta, GA 30322, USA.

Transfusion of red blood cells (RBCs) into patients with anti-donor RBC antibodies (crossmatch-incompatible transfusion) can result in lethal antibody-mediated hemolysis. Less well appreciated is the ability of anti-RBC antibodies to specifically remove their target antigen from donor RBCs without compromising cell survival or adversely affecting the transfusion recipient. In an effort to elucidate the mechanistic details of this process, we describe the first animal model of nonhemolytic antibody-induced RBC antigen loss.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!