AI Article Synopsis
- - Yeast mitochondrial DNA encodes eight major proteins, and mutations in the mitochondrial release factor mRF1 lead to the production of a new protein called V2.
- - Variations in mrf1 alleles affect how effectively the new protein, V2p, is synthesized, and the antibiotic paromomycin increases its production.
- - Analysis suggests that V2p is derived from the Var1 protein, likely resulting from a readthrough of the stop codon in the VAR1 mRNA.
Article Abstract
Yeast mitochondrial DNA codes for eight major polypeptides. Translation of he mitochondrially encoded polypeptides in strains with mutated mitochondrial release factor, mRF1, was found to result in the synthesis of a novel protein, V2. Different mrf1 alleles were associated with different efficiency of V2p synthesis. Translation of V2p was enhanced by paromomycin. Comparative analysis of peptides resulting from protease digestion indicated that V2p is a derivative of Var1p. According to our hypothesis, V2p represents a readthrough product of the natural stop codon in VAR1 mRNA.
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