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Article Synopsis
  • Standard culture methods for diagnosing infections typically use a temperature of 37 °C, but microorganisms on the ocular surface during infectious keratitis may grow better at different temperatures due to unique heat exchange features of the eye.
  • This study looked at microorganisms isolated from patients with infectious keratitis and healthy controls by growing them at both 37 °C and 24 °C, employing various identification techniques.
  • Findings revealed that certain microorganisms known as psychrotolerant can grow at lower temperatures (24 °C) and could play a role in infectious keratitis, highlighting the importance of considering temperature differences in diagnosis and treatment.
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Purpose: The current study evaluated the lid margin microbiome of keratinized lid margins of patients with chronic Stevens-Johnson syndrome (SJS) and compared it with healthy controls and historically reported lid margin microbiome of patients with meibomian gland dysfunction (MGD).

Methods: Eyelid margin swabs of 20 asymptomatic adults (mean age = 29 ± 12 years) and 10 patients with chronic SJS (mean age = 31.2 ± 14 years) with lid margin keratinization were sequenced using next generation of 16S rDNA V3 to V4 variable region.

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Purpose: Antimicrobial resistance is a global health threat, compounded by the reduction in the discovery of new antibiotics. A repurposed drugs-based approach could provide a viable alternative for the treatment of multidrug-resistant (MDR) bacterial infections. In this study, we sought to evaluate the in vitro efficacy of a novel drug combination, polymyxin B/trimethoprim (PT) + rifampin on MDR isolates from patients with bacterial keratitis in India.

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Article Synopsis
  • Infectious ulcerative keratitis is prevalent in racehorses, prompting a study to analyze the antimicrobial resistance of bacteria and fungi from the corneas of affected Japanese Thoroughbreds.
  • Out of 166 swabs from 107 cases, 85% showed bacterial or fungal infections, with Staphylococcus species being the most common bacteria, including methicillin-resistant strains (MRSA) and Aspergillus as the primary fungus.
  • Resistance to the antibiotic Ofloxacin was notably high, especially among Staphylococcus and Streptococcus isolates, while all Aspergillus strains were susceptible to voriconazole, indicating a need for more research on effective treatments for resistant infections.
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Purpose: This study aimed to investigate the effects of commercial tattoo inks used in corneal tattooing on conjunctival microbiota.

Method: This prospective case control study consisted of 125 participants divided in the following three groups: 35 patients with corneal tattoos, 40 patients with corneal leukoma, and 50 healthy subjects. Corneal tattooing was performed in all the cases in this study using a tattoo pen machine and commercial tattoo ink.

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