AI Article Synopsis
- The study highlights that ER-beta is the main estrogen receptor in the murine dorsal raphe nucleus (DRN), which is important for serotonin production.
- ER-beta and tryptophan hydroxylase (TPH) were found to be located together in serotonergic neurons, suggesting a close interaction.
- The findings show that estrogen boosts TPH1 mRNA levels through ER-beta, emphasizing its role in serotonin regulation compared to ER-alpha.
Article Abstract
Background: Distinct expression patterns of estrogen receptor (ER)-alpha and ER-beta are displayed in the murine central nervous system. ER-beta is the predominant form of the receptor expressed in the murine midbrain dorsal raphe nucleus (DRN). Tryptophan hydroxylase (TPH) is abundantly expressed in the serotonergic neurons of the DRN and is regulated by estrogen in both the monkey and the guinea pig.
Methods: In this study we used immunocytochemistry to show that ER-beta and TPH are colocalized in the serotonergic cells of the murine DRN. We utilized the ER-alpha and ER-beta gene deletion mouse models and in situ hybridization to demonstrate that ER-beta is responsible for regulating TPH1 mRNA expression.
Results: Estrogen increased TPH1 mRNA expression in the DRN of wild type and ER-alpha knockout mice (alpha-ERKO) but not ER-beta knockouts (beta-ERKO).
Conclusions: These data indicate that ER-beta is responsible for mediating estrogen regulated TPH1 expression in the murine DRN.
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| http://dx.doi.org/10.1016/j.biopsych.2005.01.014 | DOI Listing |
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