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Multilocus sequence typing directly on DNA from clinical samples and a cultured isolate to investigate linked fatal pneumococcal disease in residents of a shelter for homeless men. | LitMetric
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Multilocus sequence typing directly on DNA from clinical samples and a cultured isolate to investigate linked fatal pneumococcal disease in residents of a shelter for homeless men.

Andrew Birtles Noel McCarthy Carmen L Sheppard Harry Rutter Malcolm Guiver Elizabeth Haworth Robert C George

J Clin Microbiol

Health Protection Agency, Respiratory and Systemic Infection Laboratory, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK.

Published: April 2005

Two apparently linked fatal cases of pneumococcal meningitis were investigated. Pneumolysin PCR performed on blood and cerebrospinal fluid was positive in a culture-negative case. A second case yielded Streptococcus pneumoniae from blood culture. Multilocus sequence typing performed on DNA extracted from case 1 (specimens) and case 2 (isolate) revealed identical sequence types (ST53) substantiating the link between them.

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 Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1081336PMC
http://dx.doi.org/10.1128/JCM.43.4.2004-2008.2005DOI Listing

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