Isolation and identification of a new dicoumarin from the roots of Daphne genkwa.

Yao Xue Xue Bao

Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Xuzhou Normal University, Xuzhou 221116, China.

Published: December 2004

Aim: To investigate the chemical constituents of the roots of Daphne genkwa.

Methods: Silica gel and Sephadex LH-20 column chromatographic techniques were employed for the isolation and purification. The structure was elucidated by a combination of spectroscopic analyses.

Results: A new dicoumarin named isodaphnoretin was isolated and the structure was established as 7-hydroxy-6-methoxy-4-[(2-oxo-2H-1-benzopyran-7-yl)-oxyl]-2H-1-benzopyran-2-one.

Conclusion: Isodaphnoretin is a new compound.

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