A quantitative method based on a real-time PCR assay to enumerate Listeria monocytogenes in biofilms was developed. The specificity for L. monocytogenes of primers targeting the listeriolysin gene was demonstrated using a SYBR Green I real-time PCR assay. The number of L. monocytogenes detected growing in biofilms was 6 x 10(2) CFU/cm2.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1082516 | PMC |
| http://dx.doi.org/10.1128/AEM.71.4.2190-2194.2005 | DOI Listing |
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