AI Article Synopsis
- Prostate-specific antigen (PSA) is a biomarker for prostate diseases, with a specific sugar modification linked to its structure.
- A study used capillary electrophoresis (CE) to analyze seven different free PSA samples, revealing multiple distinct isoforms based on their sugar compositions.
- CE proved to be an effective method for quickly and reliably separating PSA into its individual isoforms.
Article Abstract
Prostate-specific antigen (PSA) is a single-chain glycoprotein that is used as a biomarker for prostate-related diseases. PSA has one known posttranslational modification, a sialylated diantennary N-linked oligosaccharide attached to the asparagine residue N45. In this study capillary electrophoresis (CE) was employed to separate the isoforms of seven commercially available free PSA samples, two of which were specialized: enzymatically active PSA and noncomplexing PSA. The free PSA samples examined migrated as four to nine distinct, highly resolved peaks, indicating the presence of several isoforms differing in their oligosaccharide compositions. Overall, the use of CE provides a rapid, reproducible method for separation of PSA into its individual isoforms.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ab.2005.01.043 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!