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[Over-expression of circNR4A1/Hsa-circ-0026352 inhibits proliferation and induces apoptosis in MCF-7 cells].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
December 2020
Beijing National Biochip Research Center Sub-center in Ningxia, General Hospital of Ningxia Medical University, College of Clinical Medicine, Ningxia Medical University, Yinchuan 750004, China. *Corresponding author, E-mail:
Objective To investigate the expression of circNR4A1/Hsa-circ-0026352 in breast tumor tissue, peripheral blood, cell lines and its effect on the proliferation and apoptosis of MCF-7 breast cancer cells. Methods Using circRNA microarray, different expression of human circRNAs was found out between the breast cancer tissues and adjacent normal tissues. The expression of circNR4A1 in breast cancer tissues, peripheral blood and breast cancer cell lines including MCF-7, HCC-1937, MDA-MB-231, T-47D and MCF-10A were detected by quantitative real-time PCR (qRT-PCR).
View Article and Find Full Text PDFSerial processing of biological reactions using flow-through microfluidic devices: coupled PCR/LDR for the detection of low-abundant DNA point mutations.
Analyst
September 2007
Department of Chemistry, Graduate School of Science, Tokyo Metropolitan University, 1-1 Minami-Ohsawa, Hachioji, Tokyo 192-0397, Japan.
We have fabricated a flow-through biochip consisting of passive elements for the analysis of single base mutations in genomic DNA using polycarbonate (PC) as the substrate. The biochip was configured to carry out two processing steps on the input sample, a primary polymerase chain reaction (PCR) followed by an allele-specific ligation detection reaction (LDR) for scoring the presence of low abundant point mutations in genomic DNA. The operation of the device was demonstrated by detecting single nucleotide polymorphisms in gene fragments (K-ras) that carry high diagnostic value for colorectal cancers.
View Article and Find Full Text PDFPolymerase chain reaction/ligase detection reaction/hybridization assays using flow-through microfluidic devices for the detection of low-abundant DNA point mutations.
Biosens Bioelectron
April 2006
Center for Bio-modular Multi-scale Systems, and Department of Chemistry, Louisiana State University, Baton Rouge, LA 70803, USA.
We have microfabricated a flow-through biochip for the analysis of single base mutations in genomic DNA using two different materials: (1) a polycarbonate (PC) chip for performing a primary polymerase chain reaction (PCR) followed by an allele-specific ligation detection reaction (LDR) and (2) a poly(methyl methacrylate) (PMMA) chip for the detection of the LDR products using a universal array platform. The operation of the device was demonstrated by detecting low-abundant DNA mutations in gene fragments (K-ras) that carry point mutations with high diagnostic value for colorectal cancers. The PC microchip was used for sequential PCR/LDR in a continuous-flow format, in which the following three steps were carried out: (1) exponential amplification of gene fragments from genomic DNA; (2) mixing of the resultant PCR product with a LDR mixture via a Y-shaped passive micromixer and (3) ligation of two primers only when the particular mutation was present in the genomic DNA.
View Article and Find Full Text PDFFabrication of DNA microarrays onto polymer substrates using UV modification protocols with integration into microfluidic platforms for the sensing of low-abundant DNA point mutations.
Methods
September 2005
Center for Bio-Modular Multi-Scale Systems, Louisiana State University, Baton Rouge, LA 70803, USA.
We describe the microfabrication and operational characteristics of a simple flow-through biochip sensor capable of detecting low abundant point mutations in K-ras oncogenes from genomic DNA, which carry high diagnostic value for colorectal cancers. The biochip consisted of an allele-specific ligase detection reaction (LDR) coupled to a universal array for interrogating multiple mutations simultaneously from a clinical sample. The integrated sensing platform was micro-manufactured from two different polymers, polycarbonate, PC, which was used for the LDRs, and poly(methyl methacrylate), PMMA, which was used to build the microarray.
View Article and Find Full Text PDFLigase detection reaction/hybridization assays using three-dimensional microfluidic networks for the detection of low-abundant DNA point mutations.
Anal Chem
May 2005
Center for Bio-Modular Multi-Scale Systems, Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana 70803, USA.
We have fabricated a flow-through biochip assembly that consisted of two different microchips: (1) a polycarbonate (PC) chip for performing an allele-specific ligation detection reaction (LDR) and (2) a poly(methyl methacrylate) (PMMA) chip for the detection of the LDR products using an universal array platform. The operation of the device was demonstrated by detecting low-abundant DNA mutations in gene fragments (K-ras) that carry point mutations with high diagnostic value for colorectal cancers. The PC microchip was used for the LDR in a continuous-flow format, in which two primers (discriminating primer that carried the complement base to the mutation being interrogated and a common primer) that flanked the point mutation and were ligated only when the particular mutation was present in the genomic DNA.
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