AI Article Synopsis

  • The study examines the ability of 7,8-DHMC, 5,7-DHMC, and gallic acid to inhibit the binding of recombinant p50 protein to DNA, finding that gallic acid is the most effective inhibitor.
  • Molecular modeling helps explain why gallic acid outperforms the other compounds in this context.
  • Additionally, the research reveals that gallic acid forms a more stable complex with zinc ions compared to the dihydroxycoumarins, impacting the p50-DNA binding process.

Article Abstract

The inhibitory effect of 7,8-dihydroxy-4-methylcoumarin (7,8-DHMC), 5,7-dihydroxy-4-methylcoumarin (5,7-DHMC), and gallic acid on the DNA binding of recombinant p50 protein and their interaction with zinc ion were studied. Electrophoretic mobility shift assay (EMSA) using p50 and biotin labeled DNA has shown that gallic acid is more effective than the dihydroxycoumarins in inhibiting the p50-DNA binding. Molecular modeling studies suggest an explanation for these observations. Effect of the addition of zinc after p50-DNA-binding inhibition by gallic acid was also studied. Chemical speciation and formation constant studies show that gallic acid forms a more stable 1:1 complex with zinc ion in comparison to the dihydroxycoumarins.

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http://dx.doi.org/10.1016/j.bioorg.2004.12.001DOI Listing

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