AI Article Synopsis
- The study examined how different vaccination schedules with a Cuban outer membrane protein-based vaccine affect the immune memory and antibody response in mice.
- Results showed that a booster shot given 7 months after initial vaccination increased antibody levels and highlighted a dose-dependent response, with more doses leading to a stronger primary response but less boost effect on bactericidal antibodies.
- It was noted that a minimum of three doses were needed to enhance the IgG avidity, and a higher number of shots expanded the specificity of the antibody response, particularly towards the PorA protein.
Article Abstract
The generation and maintenance of memory antibody response by different primary immunization schedules with the Cuban-produced outer membrane protein based vaccine was investigated in a murine model. We analyzed the duration of the antibody response (IgG-ELISA and bactericidal titer) and the effect of a booster dose on the antibody response. The IgG avidity index was determined in an attempt to find a marker for memory development. This study also included an analysis of IgG subclasses induced by primary and booster immunization. The specificity of bactericidal antibodies was investigated using local strains of the same serotype/serosubtype (4,7:P1.19,15) as the vaccine strain and mutant strains lacking major outer membrane proteins. A significant recall response was induced by a booster dose given 7 months after a primary series of 2, 3 or 4 doses of vaccine. The primary antibody response showed a positive dose-effect. In contrast, a negative dose-effect was found on the booster bactericidal antibody response. There was a significant increase in IgG1 levels after the fourth and booster doses. Three doses of vaccine were required to induce a significant increase in IgG avidity. Two injections of vaccine induced a significant antibody response to PorA protein, while 4 injections induced a larger range of specificities.
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| http://dx.doi.org/10.1016/j.femsim.2004.11.013 | DOI Listing |
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