The polysaccharide L-II was isolated and purified from the fruiting body of Lentinus edodes, which consisted of d-glucopyranose and had the molecular weight of 2.03 x 10(5) Da. We evaluated the effects of the polysaccharide L-II on the cellular immune response of Sarcoma 180-bearing mice. Mice were treated with three doses of the polysaccharide L-II (1, 5, and 10 mg/kg body weight) for 10 days. Tumor weight, relative spleen and thymus weight, delayed-type hypersensitivity (DTH) response, phagocytosis of macrophage, splenocytes proliferation were studied. Concentration of tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma) and interleukin-2 (IL-2) in mice serum were measured in control and polysaccharide groups. At the dose of 1, 5 and 10 mg/kg, a significant increase (p<0.05) in relative spleen and thymus weight, DTH, phagocytosis of macrophage was observed, as well as a significant decrease in tumor formation. The concentration of TNF-alpha, IFN-gamma in serum increased significantly in the polysaccharide groups compared with the model control group, but IL-2 not. Moreover, the polysaccharide L-II could increase NO production and catalase activity in macrophages. Results of these studies demonstrated the antitumor activity of the polysaccharide L-II on mice-transplanted sarcoma 180 was mediated by immunomodulation in inducing T-cells and macrophage-dependent immune system responses.
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http://dx.doi.org/10.1016/j.intimp.2004.11.011 | DOI Listing |
Transplant Proc
April 2017
Hepatobiliary-Pancreatic and Transplant Surgery, Mie University Graduate School of Medicine, Tsu, Japan.
Objective: The goal of this study was to evaluate whether pretransplant serum hyaluronic acid (HA) levels can predict outcomes after adult-to-adult living donor liver transplantation (LDLT).
Methods: In study I, 21 patients who underwent LDLT (March 2002-February 2004) were divided into 2 groups: the H-I group (HA ≥500 ng/mL; n = 12) and the L-I group (HA <500 ng/mL; n = 9). The influence of pretransplantation HA levels on short-term surgical outcome was investigated.
Nephrol Dial Transplant
April 2014
Department of Renal Medicine, King's College Hospital, Denmark Hill, London, UK.
Background: Rigorous data are sparse concerning the optimal route of administration and dosing strategy for iron therapy with or without concomitant erythropoiesis-stimulating agent (ESA) therapy for the management of iron deficiency anaemia in patients with non-dialysis dependent chronic kidney disease (ND-CKD).
Methods: FIND-CKD was a 56-week, open-label, multicentre, prospective, randomized three-arm study (NCT00994318) of 626 patients with ND-CKD and iron deficiency anaemia randomized to (i) intravenous (IV) ferric carboxymaltose (FCM) at an initial dose of 1000 mg iron with subsequent dosing as necessary to target a serum ferritin level of 400-600 µg/L (ii) IV FCM at an initial dose of 200 mg with subsequent dosing as necessary to target serum ferritin 100-200 µg/L or (iii) oral ferrous sulphate 200 mg iron/day. The primary end point was time to initiation of other anaemia management (ESA therapy, iron therapy other than study drug or blood transfusion) or a haemoglobin (Hb) trigger (two consecutive Hb values <10 g/dL without an increase of ≥ 0.
Dev Comp Immunol
March 2013
Laboratory of Molecular Genetics, Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan, ROC.
To date, many immunoglobulin (Ig) genes have been identified in diverse teleost species, but the contributions of different types of light chain (IgL) to the immune response remain unclear. Screening of a stimulated kidney cDNA library from orange-spotted grouper (Osg, Epinephelus coioides) resulted in the identification of 26 full Ig light chain (OsgIgL) coding sequences. These 26 OsgIgLs encoded peptides from 235 to 248 amino acid residues and could be grouped into five variable (V(L)) and four constant (C(L)) isotypes.
View Article and Find Full Text PDFJ Phys Chem B
September 2012
Unité Matériaux Et Transformations, UMR CNRS 8207, Université Lille Nord de France, USTL, 59655 Villeneuve d'Ascq, France.
Therapeutic proteins are usually conserved in glassy matrixes composed of stabilizing excipients and a small amount of water, which both control their long-term stability, and thus their potential use in medical treatments. To shed some light on the protein-matrix interactions in such systems, we performed molecular dynamics (MD) simulations on matrixes of (i) the model globular protein lysozyme (L), (ii) the well-known bioprotectant trehalose (T), and (iii) the 1:1 (in weight) lysozyme/trehalose mixture (LT), at hydration levels h of 0.0, 0.
View Article and Find Full Text PDFZhongguo Gu Shang
December 2010
Department of Orthopaedics, the Hospital Affiliated to Hubei University of TCM, Wuhan 430061, Hubei, China.
Intra-articular injection of hyaluronic acid for osteoarthritis is an old and useful method. According to recent literatures, the following four problems were reviewed in this article: 1) Mechanisms. Real-time PCR was used to detect the transforming growth factor-beta 1 (TGF-beta1), vascular endothelial growth factor (VEGF) and connective tissue growth factor (CTGF) gene expression, which were significantly increased, as well as promoted secretion and synthesis of the tissue inhibitors of metalloproteinase, thus the reaction and cartilage destruction were decreased.
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