This paper presents results from a study to develop cryopreservation procedures for apices of several strawberry genotypes. Five Fragaria x ananassa Duch. cultivars and two wild species (F. chiloensis and F. virginiana) have been screened using the encapsulation-dehydration method and/or a protocol which compromises vitrification and encapsulation-dehydration. Apices were encapsulated in an alginate gel, precultured on media containing high levels of sucrose (0.8 M, conventional protocol), or a combination of 0.4 M sucrose and 2 M glycerol. Recovery rates varied among genotypes (23-63%). The latter method reduced considerably the time needed for the cryogenic procedure by eliminating the pre-treatment with 0.8 M sucrose for 19 h prior to dehydration, as required by the conventional procedure.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Why usefulness is rarely useful.
G3 (Bethesda)
December 2024
Department of Plant Sciences, University of California Davis, Davis, CA 95616, USA.
Mate selection plays an important role in breeding programs. The Usefulness Criterion was proposed to improve mate selection, combining information on both the mean and standard deviation of the potential offspring of a cross, particularly in clonally propagated species where large family sizes are possible. Predicting the mean value of a cross is generally easier than predicting the standard deviation, especially in outbred species when the linkage of alleles is unknown and phasing is required.
View Article and Find Full Text PDFEmerging Diseases in Spain Strawberry Crops: Neopestalotiopsis Leaf and Crown Rot and Fusarium Wilt.
Plants (Basel)
December 2024
Departamento de Agronomía, ETSIA-Universidad de Sevilla, Ctra. Utrera Km 1, C.P, 41013 Seville, Spain.
In recent years, strawberry cultivation in Spain has been increasingly affected by new and re-emerging fungal diseases. The most significant emerging diseases in Spain include those caused by spp. Maharachch.
View Article and Find Full Text PDFHow to handle high subgenome sequence similarity in allopolyploid Fragaria x ananassa: linkage disequilibrium based variant filtering.
BMC Genomics
November 2024
Wageningen University and Research Plant Breeding, Wageningen, The Netherlands.
Background: The allo-octoploid Fragaria x ananassa follows disomic inheritance, yet the high sequence similarity among its subgenomes can lead to misalignment of short sequencing reads (150 bp). This misalignment results in an increased number of erroneous variants during variant calling. To accurately associate traits with the appropriate subgenome, it is essential to filter out these erroneous variants.
View Article and Find Full Text PDFFirst report of taxon × salinaslettuce (Subclade 8b hybrid) causing stem and basal rot in lettuce in North America.
Plant Dis
November 2024
USDA Agricultural Research Service, Mycology and Nematology Genetic Diversity and Biology Laboratory, 10300 Baltimore Ave, Beltsville, Maryland, United States, 20705;
Article Synopsis
- Over 55% of U.S. lettuce is produced in California, with Monterey County as the leading region, where stunted and wilted lettuce varieties were observed in 2023-2024, showing significant disease symptoms.
- Diseased plants exhibited brown lesions that developed into sunken cavities, with infection rates ranging from 5% to 75%, and lab analysis revealed the presence of Phytophthora, a harmful pathogen associated with these symptoms.
- Genetic analysis of the isolates indicated hybridization among different Phytophthora species, suggesting the emergence of a new hybrid taxon proposed as P. taxon ×salinaslettuce, which was tested for pathogenicity on various lettuce cultivars.
Phenotyping 172 strawberry genotypes for water soaking reveals a close relationship with skin water permeance.
PeerJ
September 2024
Institute for Horticultural Production Systems, Leibniz-University Hannover, Hannover, Germany.
Water soaking is a commercially important disorder of field-grown strawberries that is exacerbated by surface wetness and high humidity. The objective was to establish the effect of genotype on susceptibility to water soaking. Three greenhouse-grown model 'collections' were used comprising a total of 172 different genotypes: (1) a segregating F2 population, (2) a collection of strawberry cultivars and breeding clones, and (3) a collection of wild species.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!