AI Article Synopsis
- A 955 bp fragment of the 5'-regulatory region of the buckwheat storage globulin gene (FeLEG1) was amplified using the modified 5'-RACE approach.
- The sequence of this fragment was analyzed to identify potential cis-regulatory elements that could control gene expression in a tissue-specific and developmental manner.
- This promoter has implications for both research and practical applications in biotechnology.
Article Abstract
Using the modified rapid amplification of cDNA ends (5'-RACE) approach, a fragment containing the 955 bp long 5'-regulatory region of the buckwheat storage globulin gene (FeLEG1) has been amplified from the genomic DNA of buckwheat. The entire fragment was sequenced, and the sequence was analyzed by computer prediction of cis-regulatory elements possibly involved in tissue-specific and developmentally controlled seed storage protein gene expression. The promoter obtained might be interesting not only for fundamental research but also as a useful tool for biotechnological application.
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| http://dx.doi.org/10.1021/jf048330g | DOI Listing |
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Article Synopsis
- - The control of gene expression is crucial for genetic engineering, especially for manipulating pests like mosquitoes, but current methods lack reliable data in nonmodel insect species.
- - Researchers have found that using RNA polymerase II promoters limits options for gene expression control, prompting the need for new strategies.
- - A new systematic approach was developed to modify translation initiation sequences and 3' UTRs, successfully creating a toolbox that allows for predictable gene expression changes in mosquitoes, enhancing genetic tools for pest management.
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