The purpose of the present work was to investigate the effects of carotenoid on the spectroscopic examination of human blood serum by simulating the metabolizability of the carotenoid and porphyrin during the development of cancer and by studying the fluorescence of them. Blood serum was prepared with blood sample from elbow vein and the luminescence center of blood serum was studied using photo induced-luminescence techniques. The major factors of blood serum fluorescence were electron transitions in serological protein, carotenoid and porphyrin molecules with conjugated groups. The intensity of the fluorescence spectrum at 530 nm was enhanced while the minimum intensity at 614 nm was also enhanced with the content of carotenoid increasing, thus the value of diagnostic symbol I decreased, namely the veracity of cancer examination decreased.
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