The role of individual matrix proteins in avian eggshell calcification is poorly understood despite numerous attempts to characterize and localize their presence in the eggshell matrix. Ansocalcin, the major matrix protein from goose eggshell, was found to induce the formation of calcite crystal aggregates under in vitro. Owing to its high similarity with the chicken eggshell matrix protein ovocleidin 17 (OC-17), a comparative investigation has been carried out to understand the structure-function relationship. RP-HPLC shows that ansocalcin is the major component in extracts of goose eggshells before and after bleach treatment. However, OC-17 was observed in minute quantities in the extract of bleach-treated chicken eggshells. In vitro crystal growth experiments showed that OC-17 and ansocalcin interact differently with the calcite crystals formed. Circular dichroism, intrinsic tryptophan fluorescence, and dynamic light scattering studies showed that, under the conditions used in our experiments, OC-17 does not aggregate in solution or induce the nucleation of calcite aggregates in the concentration range used. These observations indicate that OC-17 and ansocalcin play different roles in the eggshell calcification. To our knowledge, this is the first report on the comparison of properties of homologous eggshell proteins that belong to the same phylogeny.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/bm049423+ | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Integrating proteomics and metabolomics to elucidate the regulatory mechanisms of pimpled egg production in chickens: Multi-omics analysis of the mechanism of pimpled egg formation.
Poult Sci
January 2025
Shandong Provincial Key laboratory for Livestock Germplasm Innovation & Utilization, College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Street, Taian City, Shandong Province 271018, PR China. Electronic address:
Eggshells not only protect the contents of the egg from external damage but are also a key factor influencing consumer choice, second only to price. In the later stages of egg production, the incidence of pimpled eggs significantly increases, severely affecting the hatchability and food safety of the eggs. This study compares the differences in the uterine proteomes and metabolomes of hens producing pimpled eggs and those producing normal eggs, aiming to identify the proteins and metabolites that may play a crucial role in the formation of pimpled eggs.
View Article and Find Full Text PDFEvaluation of the potential of phage phSE-5 to fight Salmonella Typhimurium in milk, liquid whole egg, and eggshell.
J Appl Microbiol
January 2025
Department of Biology and CESAM, University of Aveiro, Campus Universitário de Santiago, Aveiro 3810-193, Portugal.
Aims: This study aimed to evaluate the potential of phage phSE-5 to inactivate Salmonella enterica serovar Typhimurium in milk (at 4, 10, and 25°C), liquid whole egg, and eggshell (at 25°C for both matrices).
Methods And Results: Since the success of phage treatment in food depends on maintaining phage viability towards different food conditions, firstly the stability of phage phSE-5 at different temperatures and pHs was assessed. The effect of phage phSE-5 against S.
TMT-Based quantitative proteomic analysis reveals age-related changes in eggshell matrix proteins and their correlation with eggshell quality in Xinyang blue-shelled laying hens.
Poult Sci
December 2024
Key Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China. Electronic address:
The decline in eggshell quality with increasing hen age may be related to changes in ultrastructure and chemical composition, with matrix proteins playing key roles in these changes. However, research on blue-shelled eggs remains limited. This study investigated the effects of hen age (35, 55, 75, and 85 weeks) on the physical, mechanical, and chemical properties of eggshells in the Xinyang blue-shelled laying hens, as well as their ultrastructural and nanostructural characteristics.
View Article and Find Full Text PDFEcologic and economic motives for transforming calcium-based food wastes into sustainable value-added products: a review.
Environ Sci Pollut Res Int
January 2025
Department of Advanced Materials, Central Metallurgical R & D Institute (CMRDI), P.O. box 87, Helwan, Cairo, Egypt.
An overview of various industrial and bio-applications of unavoidable bio-waste materials reported in the literature over the last 25 years is presented in this review. Calcium-based food wastes or "unavoidable bio-wastes" are hybrid bio-composite materials, consisting of a softer organic matrix surrounding a stiff mineralized ceramic phase. A wide range of different bio-wastes that are already in use or are investigated for multipurpose applications are presented.
View Article and Find Full Text PDFSoluble Proteins From Conventional and Organic Eggshell Membranes With Different Proteomic Profiles Show Similar In Vitro Biofunctions.
J Biomed Mater Res A
January 2025
Department of Biomaterials, Institute of Clinical Dentistry, Faculty of Dentistry, University of Oslo, Oslo, Norway.
The eggshell membrane (ESM), resembling the extracellular matrix (ECM), acts as a protective barrier against bacterial invasion and offers various biofunctions due to its porous structure and protein-rich composition, such as ovalbumin, ovotransferrin, collagen, soluble protein, and antimicrobial proteins. However, the structure of ESM primarily comprises disulfide bonds and heterochains, which poses a challenge for protein solubilization/extraction. Therefore, the method of dissolving and extracting bioactive protein components from ESM has significant potential value and importance for exploring the reuse of egg waste and environmental protection.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!