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- * It was found that the RLS intensity increased with the addition of ctDNA, showing a direct relationship with its concentration, and a calibration equation was established for quantitative analysis.
- * The method demonstrated a limit of detection of 0.17 mg x L(-1) and was effectively used to measure DNA in both synthetic and real tobacco samples, yielding satisfactory results.
Article Abstract
The resonance light scattering (RLS) spectra of benzidine with deoxyribonucleic acid (ctDNA) in the presence of Sodium Dodecyl Sulfate had been studied. RLS intensity of SDS-benzidine system was enhanced by trace ctDNA and was proportional to the concentrationof ctDNA. Under optima conditions, the linear ranges of the calibration were 0.3-4 mg x L(-1), the linear regression equation was I = 37.17c (ctDNA, mg x L(-1)) + 208.9, r = 0.9992. The limit of detection were 0.17 mg x L(-1) (3delta), RSD% = 3.5%. The method had been applied to the determination of DNA in synthetic samples and gene tobacco sample, and the results were satisfactory.
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