AI Article Synopsis
- A new infectious disease surveillance method has been developed that quickly identifies both known and emerging pathogens using a combination of broad-range PCR, mass spectrometry, and base composition analysis.
- This method successfully identified and differentiated various Coronavirus species, including SARS-CoV, even when mixed with other human coronaviruses.
- With a detection sensitivity of approximately 1 PFU/mL, the technique can automate the analysis of over 900 PCR samples per day, making it applicable to various types of pathogens.
Article Abstract
We describe a new approach for infectious disease surveillance that facilitates rapid identification of known and emerging pathogens. The process uses broad-range polymerase chain reaction (PCR) to amplify nucleic acid targets from large groupings of organisms, electrospray ionization mass spectrometry for accurate mass measurements of PCR products, and base composition signature analysis to identify organisms in a sample. We demonstrate this principle by using 14 isolates of 9 diverse Coronavirus spp., including the severe acute respiratory syndrome-associated coronavirus (SARS-CoV). We show that this method could identify and distinguish between SARS and other known CoV, including the human CoV 229E and OC43, individually and in a mixture of all 3 human viruses. The sensitivity of detection, measured by using titered SARS-CoV spiked into human serum, was approximate, equals1 PFU/mL. This approach, applicable to the surveillance of bacterial, viral, fungal, or protozoal pathogens, is capable of automated analysis of >900 PCR reactions per day.
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Source |
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3298233 | PMC |
| http://dx.doi.org/10.3201/eid1103.040629 | DOI Listing |
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