A 53 year old woman developed chest pain with transient anterior subepicardial ischaemic ECG changes and a mild increase in serum myocardial enzyme concentrations. She was admitted to hospital some time later but there were no electrocardiographic signs of infarction. Echocardiography was considered to be normal. Coronary angiography showed no significant stenosis and there were no segmental wall motion abnormalities on left ventriculography. The diagnosis of a non-Q wave infarct was confirmed by myocardial scintigraphy using antimyosin monoclonal antibodies labelled with Indium 111. The site and size of the necrosis were also determined by this non-invasive investigation.
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Arch Biochem Biophys
October 2023
Department of Biological Sciences, Division of Biochemistry and Molecular Biology, University of North Texas, Denton, TX, 76203, USA. Electronic address:
The MF30 monoclonal antibody, which binds to the myosin subfragment-2 (S2), was found to increase the extent of myofibril shortening. Yet, previous observations found no effect of this antibody on actin sliding over myosin during in vitro motility assays with purified proteins in which myosin binding protein C (MyBPC) was absent. MF30 is hypothesized to enhance the availability of myosin heads (subfragment-1 or S1) to bind actin by destabilizing the myosin S2 coiled-coil and sterically blocking S2 from binding S1.
View Article and Find Full Text PDFAnn Transl Med
August 2022
Department of Rehabilitation Medicine, Catholic University of Daegu School of Medicine, Daegu, South Korea.
Background: This study aimed to examine the synergic effects of polydeoxyribonucleotide (PDRN) through extracorporeal shock wave therapy (ESWT) on atrophied calf muscles in cast-immobilized rabbit models.
Methods: Twenty male New Zealand rabbits (aged 12 weeks) were allocated into four groups. Four types of procedures [0.
ESC Heart Fail
June 2020
Cardiothoracic Surgery, Udine University Hospital, Rome, Italy.
Resistance to enzyme replacement therapy (ERT) is a major therapeutic challenge in Fabry disease (FD). Recent reports attribute to immune-mediated inflammation a main role in promoting disease progression and resistance to ERT. Aim of the study is to report a Gb3-induced auto-reactive panmyocarditis causing inefficacy of ERT and severe electrical instability, which required cardiac transplantation.
View Article and Find Full Text PDFFood Sci Anim Resour
January 2020
Graduate School of International Agricultural Technology, Seoul National University, Pyeongchang 25354, Korea.
The aim of this study was to optimize staining procedures for muscle fiber typing efficiently and rapidly in bovine and porcine skeletal muscles, such as , , , and muscles. The commercially available monoclonal anti-myosin heavy chain (MHC) antibodies and fluorescent dye-conjugated secondary antibodies were applied to immunofluorescence histology. Two different procedures, such as cocktail and serial staining, were adopted to immunofluo-rescence analysis.
View Article and Find Full Text PDFFEBS J
October 2018
Venetian Institute of Molecular Medicine (VIMM), Padova, Italy.
Different forms of myosin heavy chains (MyHCs), coded by a large family of sarcomeric MYH genes, are expressed in striated muscles. The generation of specific anti-MyHC antibodies has provided a powerful tool to define the fiber types present in skeletal muscles, their functional properties, their response to conditions that affect muscle plasticity and their changes in muscle disorders. Cardiomyocyte heterogeneity has been revealed by the serendipitous observation that different MyHCs are present in atrial and ventricular myocardium and in heart conduction tissue.
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