Antibody detection with a recombinant COOH portion of the severe acute respiratory syndrome (SARS) coronavirus nucleocapsid (N) protein, N13 (amino acids 221 to 422), was demonstrated to be more specific and sensitive than that with the full-length N protein, and an N13-based antigen-capturing enzyme-linked immunosorbent assay providing a convenient and specific test for serodiagnosis and epidemiological study of SARS was developed.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1065208 | PMC |
| http://dx.doi.org/10.1128/CDLI.12.3.474-476.2005 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Molecular mechanisms behind the generation of pro-oncogenic HIV-1 matrix protein p17 variants.
J Gen Virol
April 2024
Section of Microbiology, Department of Molecular and Translational Medicine, University of Brescia, Brescia, Italy.
Article Synopsis
- HIV-1 matrix protein p17 variants (vp17s) have amino acid insertions and exhibit stronger B cell growth and clonogenic activity than the wild-type version (refp17).
- Higher prevalence of vp17s has been observed in people living with HIV-1 who have lymphoma, suggesting a possible link to lymphomagenesis.
- The study explored the molecular mechanisms behind vp17s development, identifying mutation hot spots in the matrix protein region and demonstrating that these areas contribute to genetic recombination events that might lead to vp17s formation.
Investigation on Compositional Structures of the Organic Matter and Biomarker Distributions in Shengli Lignite.
ACS Omega
January 2024
Key Laboratory of Coal Processing and Efficient Utilization, Ministry of Education, China University of Mining and Technology, Xuzhou 221116, Jiangsu, China.
The structural characteristics of the organic matter and biomarker distributions in Shengli lignite (SL) were comprehensively studied by combining a variety of modern analytical techniques and solvent extraction/thermal dissolution. Characterization of SL with Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, solid C nuclear magnetic resonance spectroscopy and thermogravimetry showed that organic matter in SL is rich in oxygen functional groups, such as C-O, >C=O, and -COOH, and hydrogen bonds. The hydrogen bonds mainly include -OH···π, self-associated -OH, -OH···ether O, tightly bound cyclic -OH, -OH···N, -COOH dimers, and -SH···N.
View Article and Find Full Text PDFPhotochemical conversion of CO to CO by a Re complex: theoretical insights into the formation of CO and HCO from an experimentally detected monoalkyl carbonate complex.
RSC Adv
November 2021
International Research Center for Elements Science (IRCELS), Institute for Chemical Research (ICR), Kyoto University Uji Kyoto 611-0011 Japan.
Triethanolamine (TEOA) has been used for the photocatalytic reduction of CO, and the experimental studies have demonstrated that the TEOA increases the catalytic efficiency. In addition, the formation of a carbonate complex has been confirmed in the Re photocatalytic system where DMF and TEOA are used as solvents. In this study, we survey the reaction pathways of the photocatalytic conversions of CO to CO + HO and CO to CO + HCO by -Re(bpy)(CO)Br in the presence of TEOA using density functional theory (DFT) and domain-based local pair natural orbital coupled cluster approach, DLPNO-CCSD(T).
View Article and Find Full Text PDFGraphene oxide and fluorescent aptamer based novel biosensor for detection of 25-hydroxyvitamin D.
Sci Rep
December 2021
Food and Nutritional Biotechnology, National Agri-Food Biotechnology Institute (NABI), Sector-81, S.A.S. Nagar, Mohali, Punjab, India.
For maintaining the healthy metabolic status, vitamin D is a beneficial metabolite stored majorly in its pre-activated form, 25-hydroxyvitamin D (25(OH)D). Due to its important role in bone strengthening, the study was planned to quantify 25(OH)D levels in our blood. Quantification techniques for 25(OH)D are costly thus requiring a need for a low cost, and sensitive detection methods.
View Article and Find Full Text PDFEffects of the surface charge of polyamidoamine dendrimers on cellular exocytosis and the exocytosis mechanism in multidrug-resistant breast cancer cells.
J Nanobiotechnology
May 2021
Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Room 409, Yuhangtang Road 866, 310058, Hangzhou, People's Republic of China.
Background: Polyamidoamine (PAMAM) dendrimer applications have extended from tumor cells to multidrug-resistant tumor cells. However, their transportation in multidrug-resistant tumor cells remains unclear. Herein, we investigated the exocytosis rule and mechanism of PAMAM dendrimers in multidrug-resistant tumor cells.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!