To investigate the mechanism of phytochrome action in vivo, NtPHYB, AtPHYB and phyD:green fluorescent protein (GFP) were overexpressed in Nicotiana plumbaginifolia and Arabidopsis thaliana. The expression of 35S:NtPHYB:GFP and 35S:AtPHYB:GFP complemented the tobacco hgl2 and Arabidopsis phyB-9 mutations, whereas the 35S:AtPHYD:GFP only rescued the hgl2 mutant. All three fusion proteins are transported into the nucleus in all genetic backgrounds. These data indicate that AtPHYD:GFP is biologically active and functions as the main red light receptor in transgenic tobacco, and establish an experimental system for the functional analysis of this elusive photoreceptor in vivo.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1093/pcp/pci073 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The Levels of DAHP Synthase, the First Enzyme of the Shikimate Pathway, Are Related to Free Aromatic Amino Acids and Glutamine Content in Cell Cultures.
Plants (Basel)
July 2023
Laboratory of Plant Biochemistry, Department of Biology and Biotechnology, University of Pavia, 27100 Pavia, Italy.
Aromatic amino acid homeostasis was investigated in cell suspension cultures of and was related to the activity of the first enzyme in aromatic biosynthesis, 3-deoxy-D--heptulosonate-7-phosphate (DAHP) synthase. An inverse relationship was found between the intracellular content of free phenylalanine, tyrosine and tryptophan and enzyme specific activity levels, suggesting the occurrence of end-product control mechanisms. Two DAHP synthase isogenes are present in wild tobacco that showed a different expression pattern during the culture growth cycle.
View Article and Find Full Text PDF, an F-Box-Nictaba Gene, Promotes Plant Growth and Resistance to Black Shank Disease Incited by in .
Int J Mol Sci
April 2023
Key Laboratory of Horticulture Science for Southern Mountains Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China.
Black shank, a devastating disease affecting tobacco production worldwide, is caused by . However, few genes related to resistance have been reported in tobacco. Here, we identified , a gene strongly induced by race 0, with a conserved F-box motif and Nictaba (tobacco lectin) domain, in the highly resistant tobacco species .
View Article and Find Full Text PDFTranscriptomics and iTRAQ-proteomics analyses provide novel insights into the defense mechanism of black shank disease in tobacco.
Front Plant Sci
October 2022
Tobacco Breeding and Biotechnology Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, China.
Black shank disease caused by is one of the most important diseases in tobacco worldwide and can result in a devastating loss in tobacco cultivation. Many efforts have been carried out to identify the chromosome segment from containing a resistance locus carrying a gene named ; however, the gene has not been cloned, and knowledge of the potential mechanism of the gene in the resistant lines is limited. To further characterize the resistance mechanism of the gene, we first used the resistant line "RBST" and the susceptible cultivar "Honghuadajinyuan" (HD) to obtain the near-isogenic line RBS89 containing the gene from RBST.
View Article and Find Full Text PDFThe complete chloroplast genome of .
Mitochondrial DNA B Resour
January 2022
Key Laboratory of Tobacco Biotechnological Breeding, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, China.
Vivianiis 1802 is an annual herb, native to Mexico and South America. It is one of the most widely distributed tobacco species. As a wild tobacco, has provided several economically important disease-resistance genes to cultivated tobacco.
View Article and Find Full Text PDFDetection, Identification, and Molecular Characterization of a 16SrII-V Subgroup Phytoplasma Associated with .
Plant Dis
March 2022
Institute of Biochemistry, National Chung Hsing University, Taichung 402, Taiwan.
Viviani, commonly known as curl-leaved tobacco, is an annual herbaceous plant belonging to Solanaceae family. This plant is native to Mexico, South America, and parts of the Caribbean and has been reported to be present in Taiwan since 2006. In March 2021, Viviani, found in Yunlin County, Taiwan, was observed to have phyllody, virescence, and witches'-broom, which is consistent with the disease symptoms caused by phytoplasma infection.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!