Distinct fibro-vascular arrangements in the periodontal ligament of the horse.

Objective: The periodontal ligament (PDL) of the hypsodont cheek teeth of the horse meets specific requirements of tooth support: (1) to fix the tooth; (2) to withstand pressure and tension during the laterolateral mastication movement; (3) to restore the original tooth position. These requirements are assumed to be met by the periodontal collagen fiber apparatus and by the vascular system. Distinct fibro-vascular arrangements are described here in order to reveal the structural prerequisites for these distinct functions.

Design: Specimens from nine horses were processed for (1) collagen labeling with fluorescein, (2) oxytalan fiber staining, (3) immunostaining of smooth muscle actin, and (4) TEM examinations.

Results: Blood vessels were found to be integrated in the PDL in three distinct ways:

Conclusions: The sheaths formed by veil cells (type-I arrangement) isolate distinct groups of blood vessels from deformations of the surrounding tissue. In contrast, the distinct fibro-vascular arrangements of anchored blood vessels (type-II arrangement) and ballooned venules (type-III arrangement) are assumed to accept the forces generated by masticatory tooth movements. The anchored blood vessels sustain tractive forces by means of what is called a lateral compression mechanism, whereas the ballooned venules act as a hemodynamic cushion.